Restriction map for
pFN21AE3457
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# NO CUT ENZYMES ( 112 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaII BamHI BbeI BbrPI
BbvCI BcgI BfrI BglI BlpI
BpmI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstEII BstPI
BstUI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI HaeII HpaI
HphI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflIII 3731 ACATGT A^C[GA][CT]GT
Alw44I 331 GTGCAC G^TGCAC
ApaLI 331 GTGCAC G^TGCAC
AseI 3625 ATTAAT AT^TAAT
AsnI 3625 ATTAAT AT^TAAT
AviII 1944 TGCGCA TGC^GCA
BanI 217 GGTGCC G^G[CT][GA]CC
BanII 2899 GAGCTC G[GA]GC[CT]^C
BcnI 1738 CCGGG CC^[GC]GG
BglII 1168 AGATCT A^GATCT
BsaBI 223 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3943 GAGGAG GAGGAG(10/8)
Bsp1407I 3447 TGTACA T^GTACA
BspLU11I 3731 ACATGT A^CATGT
BsrDI 3504 CATTGC GCAATG(2/0)
BsrGI 3447 TGTACA T^GTACA
Bst1107I 1322 GTATAC GTA^TAC
BstBI 880 TTCGAA TT^CGAA
BstNI 3207 CCAGG CC^[AT]GG
BstZ17I 1322 GTATAC GTA^TAC
CfoI 1945 GCGC GCG^C
Cfr13I 68 GGGCC G^G[ACGT]CC
DsaI 312 CCATGG C^C[GA][CT]GG
EcoRII 3207 CCAGG ^CC[AT]GG
FauI 1024 CCCGC CCCGC(4/6)
FspI 1944 TGCGCA TGC^GCA
HapII 1738 CCGG C^CGG
HgaI 40 GACGC GACGC(5/10)
HhaI 1945 GCGC GCG^C
HinP1I 1945 GCGC G^CGC
HincII 517 GTTGAC GT[CT]^[GA]AC
HindII 517 GTTGAC GT[CT]^[GA]AC
HpaII 1738 CCGG C^CGG
MamI 223 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3884 CAATTG C^AATTG
MspA1I 713 CAGCTG C[AC]G^C[GT]G
MspI 1738 CCGG C^CGG
MunI 3884 CAATTG C^AATTG
MvaI 3207 CCAGG CC^[AT]GG
NciI 1738 CCGGG CC^[GC]GG
NcoI 312 CCATGG C^CATGG
NheI 343 GCTAGC G^CTAGC
NspV 880 TTCGAA TT^CGAA
PshBI 3625 ATTAAT AT^TAAT
PvuII 713 CAGCTG CAG^CTG
SacI 2899 GAGCTC GAGCT^C
Sau96I 68 GGGCC G^G[ACGT]CC
SfuI 880 TTCGAA TT^CGAA
SmlI 3901 CTTGAG C^T[CT][GA]AG
SphI 497 GCATGC GCATG^C
SspBI 3447 TGTACA T^GTACA
Tsp45I 3469 GTGAC ^GT[GC]AC
XbaI 3786 TCTAGA T^CTAGA
XcmI 3167 CCATGCTAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 130 GACGTC GACGT^C
520 GACGTC
AccI 109 GTAGAC GT^[AC][GT]AC
1322 GTATAC
AlwI 1477 GATCC GGATC(4/5)
478 GGATC
AvaI 2095 CCCGAG C^[CT]CG[GA]G
2452 CCCGAG
BalI 81 TGGCCA TGG^CCA
952 TGGCCA
BciVI 1887 GGATAC GTATCC(6/5)
2727 GGATAC
BclI 1224 TGATCA T^GATCA
3459 TGATCA
BsaAI 948 TACGTG [CT]AC^GT[GA]
1050 TACGTG
BsaI 3689 GAGACC GGTCTC(1/5)
1988 GGTCTC
BsmI 426 GAATGC GAATGC(1/-1)
3258 GAATGC
BsoBI 2095 CCCGAG C^[CT]CG[GA]G
2452 CCCGAG
BspHI 591 TCATGA T^CATGA
3111 TCATGA
BspMI 1663 ACCTGC ACCTGC(4/8)
3492 ACCTGC
BssKI 1738 CCGGG ^CC[ACGT]GG
3207 CCAGG
BstAPI 3508 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3640 GCAGGAGATGC
BstYI 1168 AGATCT [GA]^GATC[CT]
1476 AGATCC
EaeI 81 TGGCCA [CT]^GGCC[GA]
952 TGGCCA
FbaI 1224 TGATCA T^GATCA
3459 TGATCA
HindIII 720 AAGCTT A^AGCTT
786 AAGCTT
MflI 1168 AGATCT [GA]^GATC[CT]
1476 AGATCC
MluNI 81 TGGCCA TGG^CCA
952 TGGCCA
MscI 81 TGGCCA TGG^CCA
952 TGGCCA
RcaI 591 TCATGA T^CATGA
3111 TCATGA
SapI 1372 GAAGAGC GCTCTTC(1/4)
2998 GAAGAGC
ScrFI 1738 CCGGG CC^[ACGT]GG
3207 CCAGG
SspI 199 AATATT AAT^ATT
1268 AATATT
XhoII 1168 AGATCT [GA]^GATC[CT]
1476 AGATCC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
ApoI AspHI AvrII BbsI BbvI
BfaI BlnI BmyI BpuAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BstF5I
Cac8I DdeI DpnII DraI EarI
EcoT14I Fnu4HI FokI HaeIII Hin1I
HinfI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I