Restriction map for
pFN21AE1972
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanII BbrPI BcgI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FauI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1435 GTATAC GT^[AC][GT]AC
AccII 1257 CGCG CG^CG
AcyI 1428 GGCGCC G[GA]^CG[CT]C
AflII 403 CTTAAG C^TTAAG
AlwNI 934 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 127 CCTAAGG CC^T[ACGT]AGG
Asp700I 1055 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 486 GGATCC G^GATCC
BbeI 1428 GGCGCC GGCGC^C
BciVI 591 GTATCC GTATCC(6/5)
BclI 963 TGATCA T^GATCA
BfrI 403 CTTAAG C^TTAAG
BglII 798 AGATCT A^GATCT
BsaHI 1428 GGCGCC G[GA]^CG[CT]C
BsmI 1497 GAATGC GAATGC(1/-1)
Bst1107I 1435 GTATAC GTA^TAC
BstUI 1257 CGCG CG^CG
BstZ17I 1435 GTATAC GTA^TAC
Bsu36I 127 CCTAAGG CC^T[ACGT]AGG
DraI 691 TTTAAA TTT^AAA
Eco81I 127 CCTAAGG CC^T[ACGT]AGG
EcoRV 91 GATATC GAT^ATC
EcoT14I 482 CCAAGG C^C[AT][AT]GG
FbaI 963 TGATCA T^GATCA
HaeII 1428 GGCGCC [GA]GCGC^[CT]
Hin1I 1428 GGCGCC G[GA]^CG[CT]C
HincII 570 GTCAAC GT[CT]^[GA]AC
HindII 570 GTCAAC GT[CT]^[GA]AC
KasI 1428 GGCGCC G^GCGCC
MslI 622 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1300 CAGCTG C[AC]G^C[GT]G
MvnI 1257 CGCG CG^CG
NarI 1428 GGCGCC GG^CGCC
NspI 211 GCATGT [GA]CATG^[CT]
PvuII 1300 CAGCTG CAG^CTG
SexAI 1241 ACCTGGT A^CC[AT]GGT
SfaNI 936 GATGC GCATC(5/9)
SfoI 1428 GGCGCC GGC^GCC
StyI 482 CCAAGG C^C[AT][AT]GG
TaqI 26 TCGA T^CGA
TfiI 1164 GAATC G^A[AT]TC
TthHB8I 26 TCGA T^CGA
XmnI 1055 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 233 AAATTT [GA]^AATT[CT]
352 GAATTT
ApoI 233 AAATTT [GA]^AATT[CT]
352 GAATTT
BcnI 1293 CCCGG CC^[GC]GG
1561 CCCGG
BpmI 1273 CTCCAG CTGGAG(16/14)
698 CTGGAG
BseRI 1223 CTCCTC GAGGAG(10/8)
1445 CTCCTC
BsmFI 1017 GGGAC GGGAC(10/14)
1327 GTCCC
BsrDI 388 GCAATG GCAATG(2/0)
1000 GCAATG
Cac8I 189 GCAGGC GC[ACGT]^[ACGT]GC
463 GCAAGC
CfoI 1258 GCGC GCG^C
1429 GCGC
EarI 200 CTCTTC CTCTTC(1/4)
155 GAAGAG
HhaI 1258 GCGC GCG^C
1429 GCGC
HinP1I 1258 GCGC G^CGC
1429 GCGC
HphI 748 GGTGA GGTGA(8/7)
1008 TCACC
Ksp632I 200 CTCTTC CTCTTC(1/4)
155 GAAGAG
NciI 1293 CCCGG CC^[GC]GG
1561 CCCGG
PflMI 427 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1317 CCAGGCAGTGG
PleI 198 GACTC GAGTC(4/5)
1365 GACTC
PpuMI 1017 GGGACCT [GA]G^G[AT]CC[CT]
1503 AGGACCT
PstI 446 CTGCAG CTGCA^G
794 CTGCAG
SmlI 403 CTTAAG C^T[CT][GA]AG
550 CTCAAG
Van91I 427 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1317 CCAGGCAGTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbsI BbvCI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII