Restriction map for
pFN21AE3887
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HapII Hin1I HindIII
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AclI 320 AACGTT AA^CGTT
AflIII 909 ACATGT A^C[GA][CT]GT
AocI 524 CCTTAGG CC^T[ACGT]AGG
AspHI 94 GAGCTC G[AT]GC[AT]^C
AspI 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 162 GGACC G^G[AT]CC
BalI 815 TGGCCA TGG^CCA
BanII 94 GAGCTC G[GA]GC[CT]^C
BbsI 451 GAAGAC GAAGAC(2/6)
BciVI 860 GTATCC GTATCC(6/5)
BfaI 239 CTAG C^TAG
BmyI 94 GAGCTC G[AGT]GC[ACT]^C
BpmI 443 CTGGAG CTGGAG(16/14)
BpuAI 451 GAAGAC GAAGAC(2/6)
BsaAI 784 TACGTG [CT]AC^GT[GA]
BsaBI 799 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 640 GTGCAG GTGCAG(16/14)
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BsiYI 57 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 57 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 298 GAGAC GTCTC(1/5)
BsmI 609 GAATGC GAATGC(1/-1)
Bsp1286I 94 GAGCTC G[AGT]GC[ACT]^C
BspHI 477 TCATGA T^CATGA
BspLU11I 909 ACATGT A^CATGT
BspMI 56 ACCTGC ACCTGC(4/8)
BssKI 442 CCTGG ^CC[ACGT]GG
BstAPI 793 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 998 CATCC GGATG(2/0)
BstNI 442 CCTGG CC^[AT]GG
BstXI 1001 CCAAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 524 CCTTAGG CC^T[ACGT]AGG
Cac8I 96 GCTCGC GC[ACGT]^[ACGT]GC
DraI 915 TTTAAA TTT^AAA
EaeI 815 TGGCCA [CT]^GGCC[GA]
Eco81I 524 CCTTAGG CC^T[ACGT]AGG
EcoRII 442 CCTGG ^CC[AT]GG
FokI 998 CATCC GGATG(9/13)
HaeII 510 GGCGCT [GA]GCGC^[CT]
HgaI 231 GACGC GACGC(5/10)
HincII 355 GTTAAC GT[CT]^[GA]AC
HindII 355 GTTAAC GT[CT]^[GA]AC
HpaI 355 GTTAAC GTT^AAC
HphI 563 TCACC GGTGA(8/7)
MaeI 239 CTAG C^TAG
MaeIII 847 GTCAC ^GT[ACGT]AC
MamI 799 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 494 CAATTG C^AATTG
MluNI 815 TGGCCA TGG^CCA
MscI 815 TGGCCA TGG^CCA
MslI 998 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 494 CAATTG C^AATTG
MvaI 442 CCTGG CC^[AT]GG
PflFI 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 320 AACGTT AA^CGTT
PvuII 37 CAGCTG CAG^CTG
RcaI 477 TCATGA T^CATGA
SacI 94 GAGCTC GAGCT^C
ScrFI 442 CCTGG CC^[ACGT]GG
SfcI 989 CTACAG C^T[GA][CT]AG
SspI 77 AATATT AAT^ATT
TaqI 184 TCGA T^CGA
Tsp45I 847 GTCAC ^GT[GC]AC
Tth111I 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 184 TCGA T^CGA
XcmI 864 CCATGCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 568 AAATTC [GA]^AATT[CT]
763 AAATTC
AfaI 411 GTAC GT^AC
1014 GTAC
AlwI 21 GATCC GGATC(4/5)
339 GATCC
ApoI 568 AAATTC [GA]^AATT[CT]
763 AAATTC
BbvI 36 GCAGC GCAGC(8/12)
385 GCAGC
BclI 402 TGATCA T^GATCA
540 TGATCA
BsaJI 389 CCTTGG C^C[ACGT][ACGT]GG
832 CCTTGG
BseRI 157 GAGGAG GAGGAG(10/8)
272 GAGGAG
BstYI 20 AGATCC [GA]^GATC[CT]
338 AGATCC
CfoI 511 GCGC GCG^C
829 GCGC
Cfr13I 162 GGACC G^G[ACGT]CC
836 GGCCC
EcoT14I 389 CCTTGG C^C[AT][AT]GG
832 CCTTGG
FbaI 402 TGATCA T^GATCA
540 TGATCA
HaeIII 816 GGCC GG^CC
836 GGCC
HhaI 511 GCGC GCG^C
829 GCGC
HinP1I 511 GCGC G^CGC
829 GCGC
MaeII 321 ACGT A^CGT
785 ACGT
MflI 20 AGATCC [GA]^GATC[CT]
338 AGATCC
MspA1I 37 CAGCTG C[AC]G^C[GT]G
931 CAGCGG
NlaIV 53 GGAACC GG[ACGT]^[ACGT]CC
438 GGAGCC
NspI 900 GCATGT [GA]CATG^[CT]
909 ACATGT
PleI 520 GAGTC GAGTC(4/5)
679 GAGTC
RsaI 411 GTAC GT^AC
1014 GTAC
Sau96I 162 GGACC G^G[ACGT]CC
836 GGCCC
SfaNI 310 GATGC GCATC(5/9)
799 GATGC
StyI 389 CCTTGG C^C[AT][AT]GG
832 CCTTGG
TseI 36 GCAGC G^C[AT]GC
385 GCAGC
XhoII 20 AGATCC [GA]^GATC[CT]
338 AGATCC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI BsrI DdeI DpnII
EarI Fnu4HI HinfI ItaI Ksp632I
MboI MboII MnlI MseI MwoI
NdeII NlaIII Sau3AI TfiI Tru9I
Tsp509I TspRI