Restriction map for
pFN21AE5244
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 547 GTCGAC GT^[AC][GT]AC
Alw44I 697 GTGCAC G^TGCAC
AlwI 672 GATCC GGATC(4/5)
ApaLI 697 GTGCAC G^TGCAC
BbrPI 651 CACGTG CAC^GTG
BsaAI 651 CACGTG [CT]AC^GT[GA]
BsaI 7 GAGACC GGTCTC(1/5)
BsgI 538 CTGCAC GTGCAG(16/14)
BsiWI 603 CGTACG C^GTACG
BsmBI 250 GAGACG CGTCTC(1/5)
BssSI 442 CACGAG CACGAG(-5/-1)
BstAPI 387 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 671 AGATCC [GA]^GATC[CT]
DraII 142 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 552 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 512 TGGCCG [CT]^GGCC[GA]
EarI 82 CTCTTC CTCTTC(1/4)
EcoO109I 142 AGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 547 GTCGAC GT[CT]^[GA]AC
HindII 547 GTCGAC GT[CT]^[GA]AC
Ksp632I 82 CTCTTC CTCTTC(1/4)
KspI 587 CCGCGG CCGC^GG
MboII 83 TCTTC GAAGA(8/7)
MflI 671 AGATCC [GA]^GATC[CT]
MslI 123 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 310 GCCGGC GCC^GGC
NgoMIV 310 GCCGGC G^CCGGC
NlaIII 87 CATG CATG^
PmaCI 651 CACGTG CAC^GTG
PmlI 651 CACGTG CAC^GTG
PpuMI 142 AGGTCCC [GA]G^G[AT]CC[CT]
SacII 587 CCGCGG CCGC^GG
SalI 547 GTCGAC G^TCGAC
SapI 81 GCTCTTC GCTCTTC(1/4)
SfaNI 627 GATGC GCATC(5/9)
SmlI 100 CTCAAG C^T[CT][GA]AG
Tsp45I 59 GTCAC ^GT[GC]AC
TspRI 61 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 671 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 650 ACACGT A^C[GA][CT]GT
652 ACGTGT
AlwNI 385 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
388 CAGCTGCTG
Asp700I 159 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAAAGAGTTC
AspHI 439 GAGCAC G[AT]GC[AT]^C
697 GTGCAC
AvaII 90 GGTCC G^G[AT]CC
143 GGTCC
BbeI 282 GGCGCC GGCGC^C
576 GGCGCC
BseRI 97 CTCCTC GAGGAG(10/8)
247 GAGGAG
BsiHKAI 439 GAGCAC G[AT]GC[AT]^C
697 GTGCAC
BsmAI 7 GAGAC GTCTC(1/5)
250 GAGAC
BsmFI 144 GTCCC GGGAC(10/14)
474 GTCCC
BspMI 422 ACCTGC ACCTGC(4/8)
140 GCAGGT
BsrFI 310 GCCGGC [GA]^CCGG[CT]
686 ACCGGC
BstF5I 189 CATCC GGATG(2/0)
582 CATCC
Cfr10I 310 GCCGGC [GA]^CCGG[CT]
686 ACCGGC
DpnII 231 GATC ^GATC
672 GATC
DsaI 125 CCACGG C^C[GA][CT]GG
587 CCGCGG
FokI 189 CATCC GGATG(9/13)
582 CATCC
HgaI 208 GACGC GACGC(5/10)
252 GACGC
HinfI 298 GAGTC G^A[ACGT]TC
472 GAGTC
HphI 116 TCACC GGTGA(8/7)
122 TCACC
KasI 282 GGCGCC G^GCGCC
576 GGCGCC
MaeIII 59 GTCAC ^GT[ACGT]AC
290 GTTAC
MboI 231 GATC ^GATC
672 GATC
NarI 282 GGCGCC GG^CGCC
576 GGCGCC
NdeII 231 GATC ^GATC
672 GATC
PleI 298 GAGTC GAGTC(4/5)
472 GAGTC
PstI 226 CTGCAG CTGCA^G
519 CTGCAG
Sau3AI 231 GATC ^GATC
672 GATC
SfcI 226 CTGCAG C^T[GA][CT]AG
519 CTGCAG
SfoI 282 GGCGCC GGC^GCC
576 GGCGCC
TaqI 233 TCGA T^CGA
548 TCGA
TthHB8I 233 TCGA T^CGA
548 TCGA
XmnI 159 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiYI BslI Bsp1286I
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MaeII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PvuII RsaI Sau96I
ScrFI TseI