Restriction map for
pFN21AB9711
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BbsI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 54 GGCGCC G[GA]^CG[CT]C
Alw44I 497 GTGCAC G^TGCAC
ApaI 342 GGGCCC GGGCC^C
ApaLI 497 GTGCAC G^TGCAC
BamHI 632 GGATCC G^GATCC
BbeI 54 GGCGCC GGCGC^C
BbvCI 22 GCTGAGG CCTCAGC(-5/-2)
BcgI 125 GCAACGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 263 TGATCA T^GATCA
BfaI 652 CTAG C^TAG
BpmI 523 CTGGAG CTGGAG(16/14)
BsaHI 54 GGCGCC G[GA]^CG[CT]C
BsaWI 470 ACCGGA [AT]^CCGG[AT]
BseRI 655 GAGGAG GAGGAG(10/8)
BsmFI 60 GGGAC GGGAC(10/14)
BspDI 448 ATCGAT AT^CGAT
BstYI 632 GGATCC [GA]^GATC[CT]
ClaI 448 ATCGAT AT^CGAT
EcoT14I 1091 CCATGG C^C[AT][AT]GG
FbaI 263 TGATCA T^GATCA
FseI 933 GGCCGGCC GGCCGG^CC
HgaI 734 GCGTC GACGC(5/10)
Hin1I 54 GGCGCC G[GA]^CG[CT]C
HinfI 700 GACTC G^A[ACGT]TC
KasI 54 GGCGCC G^GCGCC
MaeI 652 CTAG C^TAG
MaeIII 503 GTAAC ^GT[ACGT]AC
MflI 632 GGATCC [GA]^GATC[CT]
MslI 564 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 54 GGCGCC GG^CGCC
NcoI 1091 CCATGG C^CATGG
PleI 700 GACTC GAGTC(4/5)
PstI 988 CTGCAG CTGCA^G
PvuII 928 CAGCTG CAG^CTG
SacI 276 GAGCTC GAGCT^C
SfcI 988 CTGCAG C^T[GA][CT]AG
SfiI 712 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 54 GGCGCC GGC^GCC
SmaI 646 CCCGGG CCC^GGG
SmlI 37 CTCAAG C^T[CT][GA]AG
SphI 725 GCATGC GCATG^C
StyI 1091 CCATGG C^C[AT][AT]GG
XhoII 632 GGATCC [GA]^GATC[CT]
XmaCI 646 CCCGGG C^CCGGG
XmaI 646 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 111 GTAC GT^AC
603 GTAC
AvaI 133 CTCGGG C^[CT]CG[GA]G
646 CCCGGG
AviII 785 TGCGCA TGC^GCA
857 TGCGCA
BbrPI 376 CACGTG CAC^GTG
865 CACGTG
BglI 713 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
743 GCCACCGCGGC
BsoBI 133 CTCGGG C^[CT]CG[GA]G
646 CCCGGG
BspMI 770 ACCTGC ACCTGC(4/8)
1082 ACCTGC
BsrI 63 ACTGG ACTGG(1/-1)
843 CCAGT
BssHII 942 GCGCGC G^CGCGC
959 GCGCGC
BstAPI 725 GCATGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1077 GCACCACCTGC
DdeI 23 CTGAG C^T[ACGT]AG
272 CTCAG
EarI 145 CTCTTC CTCTTC(1/4)
954 GAAGAG
FspI 785 TGCGCA TGC^GCA
857 TGCGCA
Ksp632I 145 CTCTTC CTCTTC(1/4)
954 GAAGAG
KspI 716 CCGCGG CCGC^GG
747 CCGCGG
NaeI 934 GCCGGC GCC^GGC
1086 GCCGGC
NgoMIV 934 GCCGGC G^CCGGC
1086 GCCGGC
NspI 725 GCATGC [GA]CATG^[CT]
782 ACATGC
PflMI 311 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCACACGCTGG
PmaCI 376 CACGTG CAC^GTG
865 CACGTG
PmlI 376 CACGTG CAC^GTG
865 CACGTG
RsaI 111 GTAC GT^AC
603 GTAC
SacII 716 CCGCGG CCGC^GG
747 CCGCGG
SapI 954 GAAGAGC GCTCTTC(1/4)
144 GCTCTTC
TaqI 149 TCGA T^CGA
449 TCGA
TthHB8I 149 TCGA T^CGA
449 TCGA
Van91I 311 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCACACGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BsaAI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI