Restriction map for
pFN21AE3994
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# NO CUT ENZYMES ( 117 enzymes) #
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AccI AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI CpoI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoT22I FseI
HincII HindII HindIII HpaI KasI
KspI MaeI MamI MfeI MluI
MslI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 604 GACGTC GACGT^C
Acc65I 1220 GGTACC G^GTACC
AccII 245 CGCG CG^CG
AlwNI 43 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1220 GGTACC G^GTACC
AspHI 1234 GAGCAC G[AT]GC[AT]^C
AspI 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 88 CCCGAG C^[CT]CG[GA]G
AviII 968 TGCGCA TGC^GCA
BanI 1220 GGTACC G^G[CT][GA]CC
BanII 66 GGGCTC G[GA]GC[CT]^C
BcgI 948 CGAGAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 701 TGATCA T^GATCA
BglI 1203 GCCCGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 229 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 229 GCTGAGC GC^T[ACGT]AGC
BsaI 667 GAGACC GGTCTC(1/5)
BsiEI 251 CGGCCG CG[GA][CT]^CG
BsiHKAI 1234 GAGCAC G[AT]GC[AT]^C
BsmBI 580 CGTCTC CGTCTC(1/5)
BsoBI 88 CCCGAG C^[CT]CG[GA]G
BspMI 643 ACCTGC ACCTGC(4/8)
BsrBI 91 GAGCGG CCGCTC(-3/-3)
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BstEII 999 GGTGACC G^GT[ACGT]ACC
BstPI 999 GGTGACC G^GT[ACGT]ACC
BstUI 245 CGCG CG^CG
CelII 229 GCTGAGC GC^T[ACGT]AGC
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
DraI 808 TTTAAA TTT^AAA
DraIII 1045 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 251 CGGCCG C^GGCCG
EarI 801 GAAGAG CTCTTC(1/4)
EclXI 251 CGGCCG C^GGCCG
Eco52I 251 CGGCCG C^GGCCG
EcoO65I 999 GGTGACC G^GT[ACGT]ACC
EcoRV 1039 GATATC GAT^ATC
FbaI 701 TGATCA T^GATCA
FspI 968 TGCGCA TGC^GCA
KpnI 1220 GGTACC GGTAC^C
Ksp632I 801 GAAGAG CTCTTC(1/4)
MvnI 245 CGCG CG^CG
PflFI 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT]
PstI 460 CTGCAG CTGCA^G
SapI 801 GAAGAGC GCTCTTC(1/4)
SfcI 460 CTGCAG C^T[GA][CT]AG
Tth111I 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 20 TCCGGA T^CCGGA
847 TCCGGA
AcyI 202 GGCGTC G[GA]^CG[CT]C
604 GACGTC
AfaI 39 GTAC GT^AC
1221 GTAC
BamHI 77 GGATCC G^GATCC
401 GGATCC
BbsI 292 GAAGAC GAAGAC(2/6)
943 GAAGAC
BpuAI 292 GAAGAC GAAGAC(2/6)
943 GAAGAC
BsaHI 202 GGCGTC G[GA]^CG[CT]C
604 GACGTC
BseAI 20 TCCGGA T^CCGGA
847 TCCGGA
BspEI 20 TCCGGA T^CCGGA
847 TCCGGA
BssSI 406 CACGAG CACGAG(-5/-1)
775 CACGAG
DraII 83 AGGACCC [GA]G^G[ACGT]CC[CT]
1076 AGGCCCT
EcoO109I 83 AGGACCC [GA]G^G[ACGT]CC[CT]
1076 AGGCCCT
EcoT14I 419 CCTTGG C^C[AT][AT]GG
477 CCTTGG
FauI 1116 CCCGC CCCGC(4/6)
246 GCGGG
HaeII 446 AGCGCC [GA]GCGC^[CT]
464 AGCGCC
Hin1I 202 GGCGTC G[GA]^CG[CT]C
604 GACGTC
MroI 20 TCCGGA T^CCGGA
847 TCCGGA
MseI 386 TTAA T^TAA
809 TTAA
PvuII 1021 CAGCTG CAG^CTG
1099 CAGCTG
RsaI 39 GTAC GT^AC
1221 GTAC
StyI 419 CCTTGG C^C[AT][AT]GG
477 CCTTGG
TaqI 35 TCGA T^CGA
1037 TCGA
TfiI 60 GATTC G^A[AT]TC
143 GAATC
Tru9I 386 TTAA T^TAA
809 TTAA
TthHB8I 35 TCGA T^CGA
1037 TCGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BbvI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI Sau3AI Sau96I
ScrFI SfaNI SmlI TseI Tsp45I
TspRI Van91I XhoII