Restriction map for
pFN21AE5923
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HapII HgaI
Hin1I HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
AflIII 470 ACACGT A^C[GA][CT]GT
AlwNI 812 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 890 GGGCCC GGGCC^C
BanI 604 GGCACC G^G[CT][GA]CC
BanII 890 GGGCCC G[GA]GC[CT]^C
BfaI 322 CTAG C^TAG
BpmI 53 CTCCAG CTGGAG(16/14)
BsgI 151 CTGCAC GTGCAG(16/14)
Bsp1407I 824 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrGI 824 TGTACA T^GTACA
BstAPI 811 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 230 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 49 GCGC GCG^C
DpnII 319 GATC ^GATC
DraIII 652 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 344 CCATGG C^C[AT][AT]GG
EcoT22I 630 ATGCAT ATGCA^T
HaeII 48 AGCGCC [GA]GCGC^[CT]
HhaI 49 GCGC GCG^C
HinP1I 49 GCGC G^CGC
HincII 199 GTTGAC GT[CT]^[GA]AC
HindII 199 GTTGAC GT[CT]^[GA]AC
MaeI 322 CTAG C^TAG
MboI 319 GATC ^GATC
MseI 211 TTAA T^TAA
MspA1I 815 CAGCTG C[AC]G^C[GT]G
NcoI 344 CCATGG C^CATGG
NdeII 319 GATC ^GATC
NsiI 630 ATGCAT ATGCA^T
PstI 714 CTGCAG CTGCA^G
PvuII 815 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
SapI 903 GCTCTTC GCTCTTC(1/4)
Sau3AI 319 GATC ^GATC
SspBI 824 TGTACA T^GTACA
StuI 701 AGGCCT AGG^CCT
StyI 344 CCATGG C^C[AT][AT]GG
Tru9I 211 TTAA T^TAA
TspRI 762 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 321 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 25 GAATTT [GA]^AATT[CT]
80 GAATTT
AocI 669 CCTGAGG CC^T[ACGT]AGG
867 CCTAAGG
ApoI 25 GAATTT [GA]^AATT[CT]
80 GAATTT
BmyI 416 GTGCCC G[AGT]GC[ACT]^C
890 GGGCCC
Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C
890 GGGCCC
BsrI 112 ACTGG ACTGG(1/-1)
505 ACTGG
BssKI 132 CCTGG ^CC[ACGT]GG
336 CCTGG
BstF5I 129 CATCC GGATG(2/0)
648 CATCC
BstNI 132 CCTGG CC^[AT]GG
336 CCTGG
Bsu36I 669 CCTGAGG CC^T[ACGT]AGG
867 CCTAAGG
Cac8I 426 GCTTGC GC[ACGT]^[ACGT]GC
660 GCCAGC
DdeI 670 CTGAG C^T[ACGT]AG
868 CTAAG
DsaI 344 CCATGG C^C[GA][CT]GG
684 CCGTGG
Eco81I 669 CCTGAGG CC^T[ACGT]AGG
867 CCTAAGG
EcoRII 132 CCTGG ^CC[AT]GG
336 CCTGG
FokI 129 CATCC GGATG(9/13)
648 CATCC
MaeII 214 ACGT A^CGT
472 ACGT
MvaI 132 CCTGG CC^[AT]GG
336 CCTGG
PpuMI 440 GGGTCCT [GA]G^G[AT]CC[CT]
751 GGGACCC
ScrFI 132 CCTGG CC^[ACGT]GG
336 CCTGG
SfaNI 234 GATGC GCATC(5/9)
258 GCATC
SfcI 714 CTGCAG C^T[GA][CT]AG
861 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AvaII BalI BbvI
BsaJI BsiYI BslI BsmFI BspMI
BsrDI Cfr13I DraII EaeI EarI
EcoO109I Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboII MluNI
MnlI MscI MslI MwoI NlaIII
NlaIV PleI RsaI Sau96I TseI
Tsp45I Tsp509I