Restriction map for
pFN21AE3092
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KspI MaeI MaeII MamI
MfeI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 537 GGTACC G^GTACC
AseI 220 ATTAAT AT^TAAT
AsnI 220 ATTAAT AT^TAAT
Asp718I 537 GGTACC G^GTACC
AspHI 562 GAGCAC G[AT]GC[AT]^C
BamHI 340 GGATCC G^GATCC
BanI 537 GGTACC G^G[CT][GA]CC
BciVI 669 GGATAC GTATCC(6/5)
BglII 404 AGATCT A^GATCT
BmyI 562 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 562 GAGCAC G[AT]GC[AT]^C
BsiYI 510 CCCAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 510 CCCAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 676 GTCTC GTCTC(1/5)
Bsp1286I 562 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 460 TGTACA T^GTACA
BsrGI 460 TGTACA T^GTACA
BssKI 337 CCAGG ^CC[ACGT]GG
BstNI 337 CCAGG CC^[AT]GG
DdeI 678 CTCAG C^T[ACGT]AG
DraI 387 TTTAAA TTT^AAA
DraII 328 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 256 GAAGAG CTCTTC(1/4)
EcoO109I 328 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 337 CCAGG ^CC[AT]GG
HgaI 525 GACGC GACGC(5/10)
HindIII 379 AAGCTT A^AGCTT
KpnI 537 GGTACC GGTAC^C
Ksp632I 256 GAAGAG CTCTTC(1/4)
MvaI 337 CCAGG CC^[AT]GG
PpuMI 328 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 220 ATTAAT AT^TAAT
PstI 467 CTGCAG CTGCA^G
ScrFI 337 CCAGG CC^[ACGT]GG
SfaNI 753 GCATC GCATC(5/9)
SfcI 467 CTGCAG C^T[GA][CT]AG
SmlI 295 CTCAAG C^T[CT][GA]AG
SspBI 460 TGTACA T^GTACA
SspI 217 AATATT AAT^ATT
TfiI 501 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 24 CCGC CCGC(-3/-1)
156 CCGC
AlwI 341 GATCC GGATC(4/5)
340 GGATC
AvaII 30 GGTCC G^G[AT]CC
329 GGACC
BbsI 93 GAAGAC GAAGAC(2/6)
765 GAAGAC
BpuAI 93 GAAGAC GAAGAC(2/6)
765 GAAGAC
BsmFI 523 GGGAC GGGAC(10/14)
201 GTCCC
BstYI 340 GGATCC [GA]^GATC[CT]
404 AGATCT
Cac8I 381 GCTTGC GC[ACGT]^[ACGT]GC
445 GCCAGC
Cfr13I 30 GGTCC G^G[ACGT]CC
329 GGACC
MflI 340 GGATCC [GA]^GATC[CT]
404 AGATCT
MwoI 136 GCCTATTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
472 GCTATCAAGGC
NlaIV 340 GGATCC GG[ACGT]^[ACGT]CC
537 GGTACC
PleI 315 GACTC GAGTC(4/5)
616 GACTC
Sau96I 30 GGTCC G^G[ACGT]CC
329 GGACC
XhoII 340 GGATCC [GA]^GATC[CT]
404 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BalI BbvI BsaJI
BsrI DpnII EaeI EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I NdeII NlaIII PvuII
RsaI Sau3AI StyI Tru9I TseI
Tsp509I TspRI