Restriction map for
pFN21AE1724
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BstAPI BstBI
BstEII BstPI BstUI BstYI Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NarI NciI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI SwaI Tru9I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 556 GTATAC GT^[AC][GT]AC
AflIII 552 ACACGT A^C[GA][CT]GT
AlwI 396 GATCC GGATC(4/5)
AlwNI 511 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 130 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 44 CTGGAG CTGGAG(16/14)
BsaAI 553 CACGTA [CT]AC^GT[GA]
BsaBI 382 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 47 GAGAC GTCTC(1/5)
Bsp1407I 444 TGTACA T^GTACA
BsrFI 66 GCCGGC [GA]^CCGG[CT]
BsrGI 444 TGTACA T^GTACA
BsrI 633 CCAGT ACTGG(1/-1)
BssSI 99 CACGAG CACGAG(-5/-1)
Bst1107I 556 GTATAC GTA^TAC
BstF5I 381 GGATG GGATG(2/0)
BstZ17I 556 GTATAC GTA^TAC
Cfr10I 66 GCCGGC [GA]^CCGG[CT]
DdeI 246 CTCAG C^T[ACGT]AG
DraII 512 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 512 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 374 GATATC GAT^ATC
EcoT14I 363 CCATGG C^C[AT][AT]GG
EcoT22I 58 ATGCAT ATGCA^T
FauI 70 GCGGG CCCGC(4/6)
FokI 381 GGATG GGATG(9/13)
HapII 67 CCGG C^CGG
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
HpaII 67 CCGG C^CGG
MaeIII 520 GTGAC ^GT[ACGT]AC
MamI 382 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 67 CCGG C^CGG
MwoI 130 GCCACTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 66 GCCGGC GCC^GGC
NcoI 363 CCATGG C^CATGG
NgoMIV 66 GCCGGC G^CCGGC
NsiI 58 ATGCAT ATGCA^T
NspI 60 GCATGT [GA]CATG^[CT]
PflFI 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 591 CCTGCAGG CCTGCA^GG
SfaNI 318 GCATC GCATC(5/9)
SmlI 661 CTTGAG C^T[CT][GA]AG
Sse8387I 591 CCTGCAGG CCTGCA^GG
SspBI 444 TGTACA T^GTACA
StyI 363 CCATGG C^C[AT][AT]GG
TaqI 467 TCGA T^CGA
Tsp45I 520 GTGAC ^GT[GC]AC
TspRI 524 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 467 TCGA T^CGA
XcmI 125 CCACAGCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 105 CCGC CCGC(-3/-1)
70 GCGG
AluI 109 AGCT AG^CT
482 AGCT
AvaII 3 GGACC G^G[AT]CC
95 GGACC
BalI 80 TGGCCA TGG^CCA
161 TGGCCA
BanII 72 GGGCTC G[GA]GC[CT]^C
275 GGGCTC
BbsI 250 GAAGAC GAAGAC(2/6)
151 GTCTTC
BciVI 624 GGATAC GTATCC(6/5)
263 GTATCC
BmyI 72 GGGCTC G[AGT]GC[ACT]^C
275 GGGCTC
BpuAI 250 GAAGAC GAAGAC(2/6)
151 GTCTTC
BsmFI 94 GGGAC GGGAC(10/14)
223 GTCCC
BsmI 539 GAATGC GAATGC(1/-1)
356 GCATTC
Bsp1286I 72 GGGCTC G[AGT]GC[ACT]^C
275 GGGCTC
BspMI 590 ACCTGC ACCTGC(4/8)
594 GCAGGT
DpnII 396 GATC ^GATC
469 GATC
DsaI 77 CCGTGG C^C[GA][CT]GG
363 CCATGG
EaeI 80 TGGCCA [CT]^GGCC[GA]
161 TGGCCA
MaeII 221 ACGT A^CGT
554 ACGT
MboI 396 GATC ^GATC
469 GATC
MluNI 80 TGGCCA TGG^CCA
161 TGGCCA
MscI 80 TGGCCA TGG^CCA
161 TGGCCA
NdeII 396 GATC ^GATC
469 GATC
Sau3AI 396 GATC ^GATC
469 GATC
TfiI 387 GAATC G^A[AT]TC
564 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI BbvI BsaJI BsiYI BslI
BssKI BstNI BstXI Cac8I Cfr13I
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MboII MnlI MslI MvaI
NlaIII NlaIV PleI PstI RsaI
Sau96I ScrFI SfcI TseI Tsp509I