##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          409  AAATTC               [GA]^AATT[CT]
ApaI          179  GGGCCC               GGGCC^C
ApoI          409  AAATTC               [GA]^AATT[CT]
AspHI          58  GAGCAC               G[AT]GC[AT]^C
AspI          591  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         590  GGACC                G^G[AT]CC
BcnI          177  CCGGG                CC^[GC]GG
BfaI          367  CTAG                 C^TAG
BseRI         580  CTCCTC               GAGGAG(10/8)
BsiHKAI        58  GAGCAC               G[AT]GC[AT]^C
BstF5I        470  GGATG                GGATG(2/0)
BstXI         527  CCATCACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI           9  GGATCT               [GA]^GATC[CT]
CfoI           95  GCGC                 GCG^C
DraI          328  TTTAAA               TTT^AAA
DsaI          465  CCGTGG               C^C[GA][CT]GG
EaeI            2  TGGCCG               [CT]^GGCC[GA]
FokI          470  GGATG                GGATG(9/13)
HapII         177  CCGG                 C^CGG
HhaI           95  GCGC                 GCG^C
HinP1I         95  GCGC                 G^CGC
HpaII         177  CCGG                 C^CGG
MaeI          367  CTAG                 C^TAG
MaeII         686  ACGT                 A^CGT
MboII         721  GAAGA                GAAGA(8/7)
MflI            9  GGATCT               [GA]^GATC[CT]
MseI          329  TTAA                 T^TAA
MslI          528  CATCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         66  CAGCTG               C[AC]G^C[GT]G
MspI          177  CCGG                 C^CGG
NciI          177  CCGGG                CC^[GC]GG
PflFI         591  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI           69  CTGCAG               CTGCA^G
PvuII          66  CAGCTG               CAG^CTG
SfcI           69  CTGCAG               C^T[GA][CT]AG
Tru9I         329  TTAA                 T^TAA
Tsp509I       410  AATT                 ^AATT
Tth111I       591  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          343  CCAGCTCTGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           9  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          420  GATCC                GGATC(4/5)
                9  GGATC               
BanII         104  GGGCTC               G[GA]GC[CT]^C
              179  GGGCCC              
BsmAI         306  GAGAC                GTCTC(1/5)
              434  GAGAC               
BspMI         112  ACCTGC               ACCTGC(4/8)
              158  ACCTGC              
DpnII          10  GATC                 ^GATC
              420  GATC                
MboI           10  GATC                 ^GATC
              420  GATC                
NdeII          10  GATC                 ^GATC
              420  GATC                
Sau3AI         10  GATC                 ^GATC
              420  GATC                
StuI          191  AGGCCT               AGG^CCT
              369  AGGCCT              
Tsp45I        457  GTCAC                ^GT[GC]AC
              692  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AluI        BbvI        BmyI        BsaJI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
EcoRII      EcoT14I     Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MaeIII      MnlI        MvaI        
MwoI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       StyI        TseI        TspRI