##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeIII      
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AfaI          673  GTAC                 GT^AC
AlwNI         643  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          101  GGGCCC               GGGCC^C
BsgI          324  CTGCAC               GTGCAG(16/14)
BsmAI         387  GAGAC                GTCTC(1/5)
BspMI         333  GCAGGT               ACCTGC(4/8)
BsrFI           8  ACCGGC               [GA]^CCGG[CT]
Cfr10I          8  ACCGGC               [GA]^CCGG[CT]
DpnII         399  GATC                 ^GATC
DrdI          652  GACCCCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HinfI         667  GATTC                G^A[ACGT]TC
MaeII          31  ACGT                 A^CGT
MboI          399  GATC                 ^GATC
NcoI          105  CCATGG               C^CATGG
NdeI          541  CATATG               CA^TATG
NdeII         399  GATC                 ^GATC
NspI          614  GCATGC               [GA]CATG^[CT]
PflMI         105  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          673  GTAC                 GT^AC
Sau3AI        399  GATC                 ^GATC
SmaI           98  CCCGGG               CCC^GGG
SphI          614  GCATGC               GCATG^C
TaqI          461  TCGA                 T^CGA
TfiI          667  GATTC                G^A[AT]TC
Tsp509I       577  AATT                 ^AATT
TthHB8I       461  TCGA                 T^CGA
Van91I        105  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          98  CCCGGG               C^CCGGG
XmaI           98  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AocI           71  CCTTAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
AvaI           98  CCCGGG               C^[CT]CG[GA]G
              602  CTCGGG              
BbvCI         125  CCTCAGC              CCTCAGC(-5/-2)
              349  GCTGAGG             
BcnI           98  CCCGG                CC^[GC]GG
               99  CCGGG               
BfaI          421  CTAG                 C^TAG
              730  CTAG                
BglI           88  GCCACCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              728  GCCTAGAGGGC         
BpmI          743  CTCCAG               CTGGAG(16/14)
              112  CTGGAG              
BsoBI          98  CCCGGG               C^[CT]CG[GA]G
              602  CTCGGG              
Bsu36I         71  CCTTAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
DraII         437  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              597  GGGGCCT             
Eco81I         71  CCTTAGG              CC^T[ACGT]AGG
              360  CCTGAGG             
EcoO109I      437  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              597  GGGGCCT             
MaeI          421  CTAG                 C^TAG
              730  CTAG                
NciI           98  CCCGG                CC^[GC]GG
               99  CCGGG               
PstI           13  CTGCAG               CTGCA^G
              238  CTGCAG              
SfaNI         268  GATGC                GCATC(5/9)
              428  GCATC               
StuI           80  AGGCCT               AGG^CCT
              524  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DsaI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MnlI        MspI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfcI        StyI        TseI        TspRI