Restriction map for
pFN21AE6461
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BglI BlnI
BpmI BsaAI BseAI BsiWI BsmBI
BsmI BspDI BspEI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MroI
MseI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tth111I
XcmI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 23 CGCG CG^CG
AcyI 135 GGCGTC G[GA]^CG[CT]C
AlwNI 610 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 715 GAGCTC G[AT]GC[AT]^C
BamHI 768 GGATCC G^GATCC
BanI 803 GGCACC G^G[CT][GA]CC
BbsI 1018 GTCTTC GAAGAC(2/6)
BbvCI 924 GCTGAGG CCTCAGC(-5/-2)
BciVI 729 GGATAC GTATCC(6/5)
BfaI 196 CTAG C^TAG
BlpI 294 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 294 GCTCAGC GC^T[ACGT]AGC
BpuAI 1018 GTCTTC GAAGAC(2/6)
BsaHI 135 GGCGTC G[GA]^CG[CT]C
BsaI 1065 GGTCTC GGTCTC(1/5)
BsaWI 148 ACCGGA [AT]^CCGG[AT]
BsgI 952 GTGCAG GTGCAG(16/14)
BsiEI 300 CGACCG CG[GA][CT]^CG
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1274 TGTACA T^GTACA
BspHI 1052 TCATGA T^CATGA
BsrBI 504 CCGCTC CCGCTC(-3/-3)
BsrDI 846 CATTGC GCAATG(2/0)
BsrFI 1252 ACCGGC [GA]^CCGG[CT]
BsrGI 1274 TGTACA T^GTACA
BssSI 124 CTCGTG CACGAG(-5/-1)
BstEII 12 GGTCACC G^GT[ACGT]ACC
BstPI 12 GGTCACC G^GT[ACGT]ACC
BstUI 23 CGCG CG^CG
BstXI 998 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 294 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1252 ACCGGC [GA]^CCGG[CT]
EarI 580 CTCTTC CTCTTC(1/4)
EcoO65I 12 GGTCACC G^GT[ACGT]ACC
HgaI 136 GCGTC GACGC(5/10)
Hin1I 135 GGCGTC G[GA]^CG[CT]C
HinfI 691 GAGTC G^A[ACGT]TC
Ksp632I 580 CTCTTC CTCTTC(1/4)
KspI 22 CCGCGG CCGC^GG
MaeI 196 CTAG C^TAG
MaeII 905 ACGT A^CGT
MvnI 23 CGCG CG^CG
NcoI 1298 CCATGG C^CATGG
NdeI 1403 CATATG CA^TATG
PaeR7I 1130 CTCGAG C^TCGAG
PflMI 811 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 691 GAGTC GAGTC(4/5)
PvuII 1234 CAGCTG CAG^CTG
RcaI 1052 TCATGA T^CATGA
SacI 715 GAGCTC GAGCT^C
SacII 22 CCGCGG CCGC^GG
SmaI 185 CCCGGG CCC^GGG
SspBI 1274 TGTACA T^GTACA
TaqI 1131 TCGA T^CGA
TthHB8I 1131 TCGA T^CGA
Van91I 811 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 195 TCTAGA T^CTAGA
XhoI 1130 CTCGAG C^TCGAG
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1115 ACATGT A^C[GA][CT]GT
1304 ACATGT
BclI 774 TGATCA T^GATCA
1049 TGATCA
BglII 317 AGATCT A^GATCT
1283 AGATCT
BsaBI 318 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
769 GATCCTGATC
BspLU11I 1115 ACATGT A^CATGT
1304 ACATGT
BsrI 389 ACTGG ACTGG(1/-1)
394 CCAGT
EcoT14I 1241 CCTTGG C^C[AT][AT]GG
1298 CCATGG
FbaI 774 TGATCA T^GATCA
1049 TGATCA
HaeII 420 GGCGCT [GA]GCGC^[CT]
471 GGCGCT
MamI 318 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
769 GATCCTGATC
PpuMI 55 AGGACCT [GA]G^G[AT]CC[CT]
683 AGGTCCC
SmlI 373 CTCAAG C^T[CT][GA]AG
1130 CTCGAG
StyI 1241 CCTTGG C^C[AT][AT]GG
1298 CCATGG
Tsp509I 226 AATT ^AATT
1365 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI XhoII