Restriction map for
pFN21AE5448
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MfeI MflI MluI MseI MslI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 86 GGTACC G^GTACC
AccIII 455 TCCGGA T^CCGGA
AciI 47 CCGC CCGC(-3/-1)
AcyI 378 GACGCC G[GA]^CG[CT]C
AlwI 825 GGATC GGATC(4/5)
AlwNI 490 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 730 GGGCCC GGGCC^C
Asp700I 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 86 GGTACC G^GTACC
AspHI 434 GTGCTC G[AT]GC[AT]^C
AvaII 910 GGTCC G^G[AT]CC
BalI 224 TGGCCA TGG^CCA
BanI 86 GGTACC G^G[CT][GA]CC
BcgI 754 GCAGGCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 75 GGATAC GTATCC(6/5)
BfaI 551 CTAG C^TAG
BsaAI 373 TACGTG [CT]AC^GT[GA]
BsaHI 378 GACGCC G[GA]^CG[CT]C
BsaJI 201 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 455 TCCGGA [AT]^CCGG[AT]
BseAI 455 TCCGGA T^CCGGA
BseRI 141 GAGGAG GAGGAG(10/8)
BsgI 181 CTGCAC GTGCAG(16/14)
BsiHKAI 434 GTGCTC G[AT]GC[AT]^C
BsmAI 475 GTCTC GTCTC(1/5)
BsmI 231 GAATGC GAATGC(1/-1)
BspEI 455 TCCGGA T^CCGGA
BsrDI 533 GCAATG GCAATG(2/0)
BstXI 344 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 122 GCGC GCG^C
EaeI 224 TGGCCA [CT]^GGCC[GA]
EcoT14I 201 CCAAGG C^C[AT][AT]GG
HapII 456 CCGG C^CGG
HhaI 122 GCGC GCG^C
Hin1I 378 GACGCC G[GA]^CG[CT]C
HinP1I 122 GCGC G^CGC
HpaII 456 CCGG C^CGG
HphI 241 GGTGA GGTGA(8/7)
KpnI 86 GGTACC GGTAC^C
MaeI 551 CTAG C^TAG
MaeII 374 ACGT A^CGT
MluNI 224 TGGCCA TGG^CCA
MroI 455 TCCGGA T^CCGGA
MscI 224 TGGCCA TGG^CCA
MspI 456 CCGG C^CGG
NdeI 608 CATATG CA^TATG
PflMI 66 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 589 GACTC GAGTC(4/5)
PstI 807 CTGCAG CTGCA^G
PvuII 903 CAGCTG CAG^CTG
SfaNI 751 GATGC GCATC(5/9)
SfcI 807 CTGCAG C^T[GA][CT]AG
SmlI 515 CTCAAG C^T[CT][GA]AG
StuI 582 AGGCCT AGG^CCT
StyI 201 CCAAGG C^C[AT][AT]GG
Van91I 66 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 201 CCAAGGGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 123 CGCG CG^CG
509 CGCG
AfaI 87 GTAC GT^AC
636 GTAC
AluI 904 AGCT AG^CT
988 AGCT
BanII 730 GGGCCC G[GA]GC[CT]^C
921 GGGCTC
BstBI 284 TTCGAA TT^CGAA
870 TTCGAA
BstUI 123 CGCG CG^CG
509 CGCG
DpnII 337 GATC ^GATC
826 GATC
EcoRI 259 GAATTC G^AATTC
648 GAATTC
HgaI 378 GACGC GACGC(5/10)
510 GCGTC
MaeIII 242 GTGAC ^GT[ACGT]AC
376 GTGAC
MboI 337 GATC ^GATC
826 GATC
MspA1I 47 CCGCTG C[AC]G^C[GT]G
903 CAGCTG
MvnI 123 CGCG CG^CG
509 CGCG
NdeII 337 GATC ^GATC
826 GATC
NspV 284 TTCGAA TT^CGAA
870 TTCGAA
RsaI 87 GTAC GT^AC
636 GTAC
Sau3AI 337 GATC ^GATC
826 GATC
SfuI 284 TTCGAA TT^CGAA
870 TTCGAA
Tsp45I 242 GTGAC ^GT[GC]AC
376 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI ApoI BbvI BmyI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I