Restriction map for
pFN21AE3413
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AccI AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MaeIII MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 766 GACGTC GACGT^C
Acc65I 503 GGTACC G^GTACC
ApaI 946 GGGCCC GGGCC^C
Asp718I 503 GGTACC G^GTACC
BalI 491 TGGCCA TGG^CCA
BbeI 37 GGCGCC GGCGC^C
BbsI 52 GTCTTC GAAGAC(2/6)
BbvCI 907 CCTCAGC CCTCAGC(-5/-2)
BcgI 81 CGAGATAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 928 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 716 AGATCT A^GATCT
BpuAI 52 GTCTTC GAAGAC(2/6)
BsmBI 768 CGTCTC CGTCTC(1/5)
Bsp1407I 369 TGTACA T^GTACA
BsrFI 977 ACCGGC [GA]^CCGG[CT]
BsrGI 369 TGTACA T^GTACA
BstAPI 324 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 977 ACCGGC [GA]^CCGG[CT]
DrdI 544 GACCAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 42 CCACGG C^C[GA][CT]GG
HphI 819 TCACC GGTGA(8/7)
KasI 37 GGCGCC G^GCGCC
KpnI 503 GGTACC GGTAC^C
MluNI 491 TGGCCA TGG^CCA
MscI 491 TGGCCA TGG^CCA
MslI 955 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 37 GGCGCC GG^CGCC
PaeR7I 446 CTCGAG C^TCGAG
SfoI 37 GGCGCC GGC^GCC
SmaI 612 CCCGGG CCC^GGG
SspBI 369 TGTACA T^GTACA
Tsp509I 800 AATT ^AATT
TspRI 693 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 446 CTCGAG C^TCGAG
XmaCI 612 CCCGGG C^CCGGG
XmaI 612 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwI 258 GGATC GGATC(4/5)
786 GGATC
AlwNI 139 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
325 CAGATGCTG
AocI 108 CCTAAGG CC^T[ACGT]AGG
410 CCTCAGG
AspHI 517 GAGCTC G[AT]GC[AT]^C
688 GAGCTC
BbrPI 67 CACGTG CAC^GTG
970 CACGTG
BcnI 612 CCCGG CC^[GC]GG
613 CCGGG
BfaI 295 CTAG C^TAG
916 CTAG
BpmI 748 CTGGAG CTGGAG(16/14)
1030 CTGGAG
BsaAI 67 CACGTG [CT]AC^GT[GA]
970 CACGTG
BsiHKAI 517 GAGCTC G[AT]GC[AT]^C
688 GAGCTC
BsmAI 73 GAGAC GTCTC(1/5)
769 GTCTC
BstXI 62 CCAAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
740 CCATGCCCCTGG
Bsu36I 108 CCTAAGG CC^T[ACGT]AGG
410 CCTCAGG
Eco81I 108 CCTAAGG CC^T[ACGT]AGG
410 CCTCAGG
EcoNI 93 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
747 CCTGGAGGAGG
EcoT14I 839 CCAAGG C^C[AT][AT]GG
932 CCAAGG
FauI 533 CCCGC CCCGC(4/6)
147 GCGGG
HaeII 37 GGCGCC [GA]GCGC^[CT]
406 AGCGCC
HgaI 551 GCGTC GACGC(5/10)
912 GCGTC
MaeI 295 CTAG C^TAG
916 CTAG
MboII 53 TCTTC GAAGA(8/7)
863 TCTTC
MseI 155 TTAA T^TAA
725 TTAA
NciI 612 CCCGG CC^[GC]GG
613 CCGGG
PleI 449 GAGTC GAGTC(4/5)
991 GAGTC
PmaCI 67 CACGTG CAC^GTG
970 CACGTG
PmlI 67 CACGTG CAC^GTG
970 CACGTG
PstI 163 CTGCAG CTGCA^G
322 CTGCAG
SacI 517 GAGCTC GAGCT^C
688 GAGCTC
ScaI 8 AGTACT AGT^ACT
857 AGTACT
SexAI 485 ACCAGGT A^CC[AT]GGT
496 ACCAGGT
SfcI 163 CTGCAG C^T[GA][CT]AG
322 CTGCAG
StyI 839 CCAAGG C^C[AT][AT]GG
932 CCAAGG
TaqI 447 TCGA T^CGA
554 TCGA
TfiI 903 GATTC G^A[AT]TC
1014 GATTC
Tru9I 155 TTAA T^TAA
725 TTAA
TthHB8I 447 TCGA T^CGA
554 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
AvaII BanI BanII BbvI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MboI MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TseI Van91I XhoII