Restriction map for
pFN21AE2724
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# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvI
BcgI BclI BfrI BglI BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
HphI ItaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MslI MspA1I MunI MvnI MwoI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AluI 343 AGCT AG^CT
AlwNI 122 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 292 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 525 GGACC G^G[AT]CC
BalI 432 TGGCCA TGG^CCA
BbvCI 265 GCTGAGG CCTCAGC(-5/-2)
BciVI 421 GGATAC GTATCC(6/5)
BcnI 418 CCGGG CC^[GC]GG
BfaI 565 CTAG C^TAG
BglII 549 AGATCT A^GATCT
BpmI 407 CTCCAG CTGGAG(16/14)
BsmAI 136 GAGAC GTCTC(1/5)
BsrI 54 ACTGG ACTGG(1/-1)
BstYI 549 AGATCT [GA]^GATC[CT]
CfoI 227 GCGC GCG^C
DpnII 550 GATC ^GATC
DraII 524 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 235 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 432 TGGCCA [CT]^GGCC[GA]
EarI 520 GAAGAG CTCTTC(1/4)
EcoO109I 524 AGGACCT [GA]G^G[ACGT]CC[CT]
HapII 418 CCGG C^CGG
HhaI 227 GCGC GCG^C
HinP1I 227 GCGC G^CGC
HpaII 418 CCGG C^CGG
Ksp632I 520 GAAGAG CTCTTC(1/4)
MaeI 565 CTAG C^TAG
MboI 550 GATC ^GATC
MflI 549 AGATCT [GA]^GATC[CT]
MluNI 432 TGGCCA TGG^CCA
MscI 432 TGGCCA TGG^CCA
MspI 418 CCGG C^CGG
NciI 418 CCGGG CC^[GC]GG
NdeI 362 CATATG CA^TATG
NdeII 550 GATC ^GATC
PpuMI 524 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 550 GATC ^GATC
TaqI 290 TCGA T^CGA
TthHB8I 290 TCGA T^CGA
XhoII 549 AGATCT [GA]^GATC[CT]
XmnI 292 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 63 GAATTT [GA]^AATT[CT]
272 AAATTC
ApoI 63 GAATTT [GA]^AATT[CT]
272 AAATTC
BseRI 381 GAGGAG GAGGAG(10/8)
384 GAGGAG
BstNI 255 CCAGG CC^[AT]GG
501 CCTGG
Cfr13I 443 GGCCC G^G[ACGT]CC
525 GGACC
EcoRII 255 CCAGG ^CC[AT]GG
501 CCTGG
HaeIII 433 GGCC GG^CC
443 GGCC
MaeIII 31 GTGAC ^GT[ACGT]AC
589 GTGAC
MvaI 255 CCAGG CC^[AT]GG
501 CCTGG
PleI 412 GACTC GAGTC(4/5)
124 GAGTC
Sau96I 443 GGCCC G^G[ACGT]CC
525 GGACC
TfiI 20 GAATC G^A[AT]TC
357 GATTC
Tsp45I 31 GTGAC ^GT[GC]AC
589 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AfaI BssKI BstF5I DdeI FokI
HinfI MboII MnlI MseI NlaIII
RsaI ScrFI Tru9I Tsp509I TspRI