Restriction map for
pFN21AE3239
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstPI
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoT14I EcoT22I FbaI FseI FspI
HindIII KpnI MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 852 GTATAC GT^[AC][GT]AC
AocI 1631 CCTCAGG CC^T[ACGT]AGG
ApaI 13 GGGCCC GGGCC^C
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsmAI 1357 GTCTC GTCTC(1/5)
BspHI 627 TCATGA T^CATGA
BspLU11I 1139 ACATGT A^CATGT
BspMI 679 ACCTGC ACCTGC(4/8)
Bst1107I 852 GTATAC GTA^TAC
BstYI 711 GGATCT [GA]^GATC[CT]
BstZ17I 852 GTATAC GTA^TAC
Bsu36I 1631 CCTCAGG CC^T[ACGT]AGG
DraII 13 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 41 CGGCCG [CT]^GGCC[GA]
EagI 41 CGGCCG C^GGCCG
EarI 1274 CTCTTC CTCTTC(1/4)
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 1631 CCTCAGG CC^T[ACGT]AGG
EcoO109I 13 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1327 GAATTC G^AATTC
EcoRV 959 GATATC GAT^ATC
HgaI 66 GACGC GACGC(5/10)
HpaI 915 GTTAAC GTT^AAC
Ksp632I 1274 CTCTTC CTCTTC(1/4)
MflI 711 GGATCT [GA]^GATC[CT]
NaeI 284 GCCGGC GCC^GGC
NgoMIV 284 GCCGGC G^CCGGC
NspI 1139 ACATGT [GA]CATG^[CT]
PaeR7I 520 CTCGAG C^TCGAG
PstI 303 CTGCAG CTGCA^G
PvuII 1249 CAGCTG CAG^CTG
RcaI 627 TCATGA T^CATGA
ScaI 524 AGTACT AGT^ACT
SfcI 303 CTGCAG C^T[GA][CT]AG
XhoI 520 CTCGAG C^TCGAG
XhoII 711 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 202 TCCGGA T^CCGGA
228 TCCGGA
AcsI 880 AAATTC [GA]^AATT[CT]
1327 GAATTC
AflIII 892 ACACGT A^C[GA][CT]GT
1139 ACATGT
ApoI 880 AAATTC [GA]^AATT[CT]
1327 GAATTC
Asp700I 1037 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1454 GAATGATTTC
AvaII 414 GGACC G^G[AT]CC
1081 GGTCC
BbeI 172 GGCGCC GGCGC^C
505 GGCGCC
BbsI 631 GAAGAC GAAGAC(2/6)
511 GTCTTC
BbvCI 486 CCTCAGC CCTCAGC(-5/-2)
55 GCTGAGG
BpuAI 631 GAAGAC GAAGAC(2/6)
511 GTCTTC
BsaWI 202 TCCGGA [AT]^CCGG[AT]
228 TCCGGA
BseAI 202 TCCGGA T^CCGGA
228 TCCGGA
BseRI 58 GAGGAG GAGGAG(10/8)
1306 GAGGAG
BsgI 1252 CTGCAC GTGCAG(16/14)
1509 GTGCAG
BspEI 202 TCCGGA T^CCGGA
228 TCCGGA
BsrFI 284 GCCGGC [GA]^CCGG[CT]
665 ACCGGC
BsrI 1345 CCAGT ACTGG(1/-1)
1544 CCAGT
BssSI 73 CACGAG CACGAG(-5/-1)
1145 CACGAG
BstXI 18 CCATAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1258 CCAAAAGGCTGG
Cfr10I 284 GCCGGC [GA]^CCGG[CT]
665 ACCGGC
HincII 915 GTTAAC GT[CT]^[GA]AC
1522 GTTGAC
HindII 915 GTTAAC GT[CT]^[GA]AC
1522 GTTGAC
KasI 172 GGCGCC G^GCGCC
505 GGCGCC
KspI 399 CCGCGG CCGC^GG
470 CCGCGG
MroI 202 TCCGGA T^CCGGA
228 TCCGGA
NarI 172 GGCGCC GG^CGCC
505 GGCGCC
SacI 224 GAGCTC GAGCT^C
1340 GAGCTC
SacII 399 CCGCGG CCGC^GG
470 CCGCGG
SfaNI 127 GATGC GCATC(5/9)
1235 GCATC
SfiI 322 GGCCGACACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
343 GGCCTGGGCGGCC
SfoI 172 GGCGCC GGC^GCC
505 GGCGCC
SmlI 520 CTCGAG C^T[CT][GA]AG
1287 CTCAAG
TaqI 521 TCGA T^CGA
776 TCGA
TfiI 1117 GATTC G^A[AT]TC
1482 GATTC
TthHB8I 521 TCGA T^CGA
776 TCGA
XmnI 1037 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1454 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SmaI Tru9I TseI Tsp45I Tsp509I
TspRI XmaCI XmaI