Restriction map for
pFN21AE6552
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MfeI MflI
MluI MluNI MscI MseI MslI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tru9I Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 190 GTATAC GT^[AC][GT]AC
AccIII 15 TCCGGA T^CCGGA
AciI 30 GCGG CCGC(-3/-1)
Asp700I 343 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 89 CTCGGG C^[CT]CG[GA]G
BanII 84 GAGCCC G[GA]GC[CT]^C
BclI 372 TGATCA T^GATCA
BlpI 81 GCTGAGC GC^T[ACGT]AGC
BmyI 84 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 81 GCTGAGC GC^T[ACGT]AGC
BsaBI 373 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 15 TCCGGA [AT]^CCGG[AT]
BseAI 15 TCCGGA T^CCGGA
BsiYI 164 CCCCTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 164 CCCCTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 334 GAGAC GTCTC(1/5)
BsoBI 89 CTCGGG C^[CT]CG[GA]G
Bsp1286I 84 GAGCCC G[AGT]GC[ACT]^C
BspEI 15 TCCGGA T^CCGGA
BsrFI 96 GCCGGT [GA]^CCGG[CT]
BsrI 337 ACTGG ACTGG(1/-1)
Bst1107I 190 GTATAC GTA^TAC
BstAPI 36 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 18 GGATG GGATG(2/0)
BstZ17I 190 GTATAC GTA^TAC
CelII 81 GCTGAGC GC^T[ACGT]AGC
Cfr10I 96 GCCGGT [GA]^CCGG[CT]
DpnII 373 GATC ^GATC
DraII 120 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 183 CCATGG C^C[GA][CT]GG
EcoO109I 120 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 372 TGATCA T^GATCA
FokI 18 GGATG GGATG(9/13)
HaeIII 122 GGCC GG^CC
HphI 319 GGTGA GGTGA(8/7)
MamI 373 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 373 GATC ^GATC
MboII 114 GAAGA GAAGA(8/7)
MroI 15 TCCGGA T^CCGGA
MspA1I 40 CAGCTG C[AC]G^C[GT]G
NcoI 183 CCATGG C^CATGG
NdeI 281 CATATG CA^TATG
NdeII 373 GATC ^GATC
PstI 34 CTGCAG CTGCA^G
PvuII 40 CAGCTG CAG^CTG
Sau3AI 373 GATC ^GATC
SfcI 34 CTGCAG C^T[GA][CT]AG
SspI 309 AATATT AAT^ATT
XmnI 343 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 37 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
76 CAGGAGCTG
AvaII 72 GGACC G^G[AT]CC
180 GGTCC
BpmI 331 CTGGAG CTGGAG(16/14)
338 CTGGAG
BssKI 75 CCAGG ^CC[ACGT]GG
316 CCTGG
BstNI 75 CCAGG CC^[AT]GG
316 CCTGG
DdeI 52 CTGAG C^T[ACGT]AG
82 CTGAG
EcoRII 75 CCAGG ^CC[AT]GG
316 CCTGG
EcoT14I 166 CCTTGG C^C[AT][AT]GG
183 CCATGG
HapII 16 CCGG C^CGG
97 CCGG
HpaII 16 CCGG C^CGG
97 CCGG
MnlI 88 CCTC CCTC(7/6)
171 GAGG
MspI 16 CCGG C^CGG
97 CCGG
MvaI 75 CCAGG CC^[AT]GG
316 CCTGG
NlaIV 93 GGAGCC GG[ACGT]^[ACGT]CC
120 GGGGCC
ScrFI 75 CCAGG CC^[ACGT]GG
316 CCTGG
StyI 166 CCTTGG C^C[AT][AT]GG
183 CCATGG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI Cfr13I Fnu4HI ItaI MwoI
NlaIII RsaI Sau96I TseI Tsp509I