Restriction map for
pFN21AE1675
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp45I TspRI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 405 GTAC GT^AC
AhdI 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 37 GTGCTC G[AT]GC[AT]^C
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
BpmI 355 CTGGAG CTGGAG(16/14)
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsaI 304 GAGACC GGTCTC(1/5)
BseRI 190 GAGGAG GAGGAG(10/8)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsiYI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 21 ACTGG ACTGG(1/-1)
BstYI 517 AGATCC [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 352 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
HphI 384 GGTGA GGTGA(8/7)
MaeIII 98 GTAAC ^GT[ACGT]AC
MflI 517 AGATCC [GA]^GATC[CT]
MspA1I 343 CAGCTG C[AC]G^C[GT]G
MwoI 59 GCTCTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PvuII 343 CAGCTG CAG^CTG
RcaI 512 TCATGA T^CATGA
RsaI 405 GTAC GT^AC
SfaNI 369 GATGC GCATC(5/9)
SmlI 221 CTCAAG C^T[CT][GA]AG
TaqI 329 TCGA T^CGA
TthHB8I 329 TCGA T^CGA
XbaI 312 TCTAGA T^CTAGA
XhoII 517 AGATCC [GA]^GATC[CT]
XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 178 GAATTC [GA]^AATT[CT]
319 GAATTC
AlwI 249 GATCC GGATC(4/5)
518 GATCC
ApoI 178 GAATTC [GA]^AATT[CT]
319 GAATTC
BbvI 342 GCAGC GCAGC(8/12)
292 GCTGC
BfaI 31 CTAG C^TAG
313 CTAG
BsmAI 304 GAGAC GTCTC(1/5)
393 GTCTC
BstF5I 417 CATCC GGATG(2/0)
300 GGATG
Cfr13I 27 GGCCC G^G[ACGT]CC
107 GGGCC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
EarI 268 CTCTTC CTCTTC(1/4)
555 GAAGAG
EcoRI 178 GAATTC G^AATTC
319 GAATTC
Fnu4HI 292 GCTGC GC^[ACGT]GC
342 GCAGC
FokI 417 CATCC GGATG(9/13)
300 GGATG
ItaI 292 GCTGC GC^[ACGT]GC
342 GCAGC
Ksp632I 268 CTCTTC CTCTTC(1/4)
555 GAAGAG
MaeI 31 CTAG C^TAG
313 CTAG
Sau96I 27 GGCCC G^G[ACGT]CC
107 GGGCC
TfiI 137 GAATC G^A[AT]TC
446 GAATC
TseI 292 GCTGC G^C[AT]GC
342 GCAGC
Tsp509I 179 AATT ^AATT
320 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsaJI BssKI BstNI DpnII
EcoRII HaeIII HinfI MboI MboII
MnlI MvaI NdeII NlaIII PleI
Sau3AI ScrFI