##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BpmI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tsp45I      
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          319  AAATTT               [GA]^AATT[CT]
AluI           43  AGCT                 AG^CT
AlwNI         340  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          319  AAATTT               [GA]^AATT[CT]
Asp700I       432  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          37  GTGCTC               G[AT]GC[AT]^C
BbvI          292  GCTGC                GCAGC(8/12)
BlpI           39  GCTCAGC              GC^T[ACGT]AGC
BmyI           37  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I       39  GCTCAGC              GC^T[ACGT]AGC
BseRI         190  GAGGAG               GAGGAG(10/8)
BsiHKAI        37  GTGCTC               G[AT]GC[AT]^C
BsmAI         391  GAGAC                GTCTC(1/5)
BsmFI         123  GGGAC                GGGAC(10/14)
Bsp1286I       37  GTGCTC               G[AGT]GC[ACT]^C
BspHI         512  TCATGA               T^CATGA
BsrI          207  CCAGT                ACTGG(1/-1)
BstF5I        417  CATCC                GGATG(2/0)
BstYI         517  AGATCC               [GA]^GATC[CT]
CelII          39  GCTCAGC              GC^T[ACGT]AGC
DraII         106  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI           26  TGGCCG               [CT]^GGCC[GA]
EcoO109I      106  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Fnu4HI        292  GCTGC                GC^[ACGT]GC
FokI          417  CATCC                GGATG(9/13)
ItaI          292  GCTGC                GC^[ACGT]GC
MflI          517  AGATCC               [GA]^GATC[CT]
MseI          553  TTAA                 T^TAA
NspI          361  GCATGT               [GA]CATG^[CT]
PflMI          87  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          512  TCATGA               T^CATGA
SmlI          221  CTCAAG               C^T[CT][GA]AG
Tru9I         553  TTAA                 T^TAA
TseI          292  GCTGC                G^C[AT]GC
Tsp509I       320  AATT                 ^AATT
TspRI         327  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I         87  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         517  AGATCC               [GA]^GATC[CT]
XmnI          432  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          249  GATCC                GGATC(4/5)
              518  GATCC               
BfaI           64  CTAG                 C^TAG
              313  CTAG                
BsiYI          87  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              183  CCCCCAGGAGG         
BslI           87  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              183  CCCCCAGGAGG         
Cfr13I          3  GGCCC                G^G[ACGT]CC
              107  GGGCC               
DdeI           40  CTCAG                C^T[ACGT]AG
               80  CTCAG               
EarI          268  CTCTTC               CTCTTC(1/4)
              388  GAAGAG              
Ksp632I       268  CTCTTC               CTCTTC(1/4)
              388  GAAGAG              
MaeI           64  CTAG                 C^TAG
              313  CTAG                
Sau96I          3  GGCCC                G^G[ACGT]CC
              107  GGGCC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

BsaJI       BssKI       BstNI       DpnII       EcoRII      
HaeIII      HinfI       MboI        MboII       MnlI        
MvaI        NdeII       NlaIII      PleI        Sau3AI      
ScrFI       TfiI