Restriction map for
pFN21AE6529
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BbvI BciVI
BclI BfaI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 291 GTCTAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AflII 471 CTTAAG C^TTAAG
AluI 92 AGCT AG^CT
AvaI 266 CCCGGG C^[CT]CG[GA]G
BbsI 575 GTCTTC GAAGAC(2/6)
BcgI 196 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 471 CTTAAG C^TTAAG
BpmI 147 CTGGAG CTGGAG(16/14)
BpuAI 575 GTCTTC GAAGAC(2/6)
BsmFI 21 GTCCC GGGAC(10/14)
BsmI 429 GAATGC GAATGC(1/-1)
BsoBI 266 CCCGGG C^[CT]CG[GA]G
Bsp1407I 547 TGTACA T^GTACA
BspHI 423 TCATGA T^CATGA
BspMI 101 GCAGGT ACCTGC(4/8)
BsrDI 567 CATTGC GCAATG(2/0)
BsrGI 547 TGTACA T^GTACA
BssSI 59 CTCGTG CACGAG(-5/-1)
BstBI 238 TTCGAA TT^CGAA
BstNI 86 CCAGG CC^[AT]GG
BstUI 5 CGCG CG^CG
Cac8I 245 GCCCGC GC[ACGT]^[ACGT]GC
Cfr13I 264 GGCCC G^G[ACGT]CC
DpnII 614 GATC ^GATC
DraI 592 TTTAAA TTT^AAA
DsaI 4 CCGCGG C^C[GA][CT]GG
EaeI 135 CGGCCA [CT]^GGCC[GA]
EarI 272 GAAGAG CTCTTC(1/4)
EcoRII 86 CCAGG ^CC[AT]GG
EcoT14I 372 CCTTGG C^C[AT][AT]GG
FauI 246 CCCGC CCCGC(4/6)
HinfI 38 GACTC G^A[ACGT]TC
Ksp632I 272 GAAGAG CTCTTC(1/4)
KspI 4 CCGCGG CCGC^GG
MaeII 450 ACGT A^CGT
MboI 614 GATC ^GATC
MslI 424 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 4 CCGCGG C[AC]G^C[GT]G
MvaI 86 CCAGG CC^[AT]GG
MvnI 5 CGCG CG^CG
NdeII 614 GATC ^GATC
NspI 571 GCATGT [GA]CATG^[CT]
NspV 238 TTCGAA TT^CGAA
PleI 38 GACTC GAGTC(4/5)
RcaI 423 TCATGA T^CATGA
SacII 4 CCGCGG CCGC^GG
Sau3AI 614 GATC ^GATC
Sau96I 264 GGCCC G^G[ACGT]CC
SfaNI 492 GCATC GCATC(5/9)
SfcI 222 CTACAG C^T[GA][CT]AG
SfuI 238 TTCGAA TT^CGAA
SmaI 266 CCCGGG CCC^GGG
SmlI 471 CTTAAG C^T[CT][GA]AG
SspBI 547 TGTACA T^GTACA
StuI 200 AGGCCT AGG^CCT
StyI 372 CCTTGG C^C[AT][AT]GG
Tsp45I 333 GTCAC ^GT[GC]AC
XmaCI 266 CCCGGG C^CCGGG
XmaI 266 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 388 GAATTT [GA]^AATT[CT]
436 AAATTT
AfaI 105 GTAC GT^AC
548 GTAC
ApoI 388 GAATTT [GA]^AATT[CT]
436 AAATTT
BseRI 10 CTCCTC GAGGAG(10/8)
166 GAGGAG
BstF5I 496 CATCC GGATG(2/0)
544 GGATG
FokI 496 CATCC GGATG(9/13)
544 GGATG
HapII 262 CCGG C^CGG
267 CCGG
HpaII 262 CCGG C^CGG
267 CCGG
HphI 111 GGTGA GGTGA(8/7)
367 TCACC
MaeIII 333 GTCAC ^GT[ACGT]AC
625 GTAAC
MspI 262 CCGG C^CGG
267 CCGG
MwoI 71 GCGACATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
256 GCTTCCCCGGC
NlaIV 8 GGCTCC GG[ACGT]^[ACGT]CC
149 GGAGCC
RsaI 105 GTAC GT^AC
548 GTAC
TspRI 81 CAGTG CA[GC]TG[ACGT][ACGT]^
287 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BcnI BsaJI BsiYI BslI
BsrI BssKI DdeI Fnu4HI HaeIII
ItaI MboII MnlI MseI NciI
NlaIII ScrFI TaqI Tru9I Tsp509I
TthHB8I