Restriction map for
pFN21AE3231
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccIII AclI AcsI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BamHI BbrPI
BbsI BcgI BciVI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT22I HincII HindII
HindIII HpaI Ksp632I MamI MfeI
MluI MroI MunI NdeI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 107 GGTACC G^GTACC
AcyI 90 GGCGCC G[GA]^CG[CT]C
AflII 1392 CTTAAG C^TTAAG
AocI 1437 CCTCAGG CC^T[ACGT]AGG
Asp718I 107 GGTACC G^GTACC
AspI 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 821 TGCGCA TGC^GCA
BalI 1344 TGGCCA TGG^CCA
BbeI 90 GGCGCC GGCGC^C
BbvCI 982 CCTCAGC CCTCAGC(-5/-2)
BclI 1478 TGATCA T^GATCA
BfrI 1392 CTTAAG C^TTAAG
BglI 1247 GCCTGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 90 GGCGCC G[GA]^CG[CT]C
BsaI 335 GAGACC GGTCTC(1/5)
BsgI 361 GTGCAG GTGCAG(16/14)
BsrBI 57 CCGCTC CCGCTC(-3/-3)
BstAPI 881 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 779 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1437 CCTCAGG CC^T[ACGT]AGG
Eco81I 1437 CCTCAGG CC^T[ACGT]AGG
EcoNI 1368 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1478 TGATCA T^GATCA
FseI 502 GGCCGGCC GGCCGG^CC
FspI 821 TGCGCA TGC^GCA
Hin1I 90 GGCGCC G[GA]^CG[CT]C
KasI 90 GGCGCC G^GCGCC
KpnI 107 GGTACC GGTAC^C
MaeII 1258 ACGT A^CGT
MluNI 1344 TGGCCA TGG^CCA
MscI 1344 TGGCCA TGG^CCA
MslI 780 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 90 GGCGCC GG^CGCC
NcoI 767 CCATGG C^CATGG
NheI 720 GCTAGC G^CTAGC
NotI 268 GCGGCCGC GC^GGCCGC
NspI 131 GCATGC [GA]CATG^[CT]
PflFI 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 90 GGCGCC GGC^GCC
SphI 131 GCATGC GCATG^C
TaqI 959 TCGA T^CGA
Tth111I 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 959 TCGA T^CGA
XcmI 398 CCATGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 108 GTAC GT^AC
622 GTAC
AlwI 1167 GGATC GGATC(4/5)
1209 GGATC
ApaI 219 GGGCCC GGGCC^C
316 GGGCCC
BseRI 802 GAGGAG GAGGAG(10/8)
1075 GAGGAG
BsmAI 335 GAGAC GTCTC(1/5)
615 GTCTC
BstF5I 603 CATCC GGATG(2/0)
861 CATCC
BstYI 1167 GGATCT [GA]^GATC[CT]
1209 GGATCT
DrdI 480 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
706 GACGCTCTGGTC
FokI 603 CATCC GGATG(9/13)
861 CATCC
KspI 49 CCGCGG CCGC^GG
230 CCGCGG
MboII 534 GAAGA GAAGA(8/7)
1170 TCTTC
MflI 1167 GGATCT [GA]^GATC[CT]
1209 GGATCT
MseI 1393 TTAA T^TAA
1433 TTAA
NaeI 503 GCCGGC GCC^GGC
1194 GCCGGC
NgoMIV 503 GCCGGC G^CCGGC
1194 GCCGGC
PflMI 744 CCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGGTCCTGG
PstI 118 CTGCAG CTGCA^G
967 CTGCAG
RsaI 108 GTAC GT^AC
622 GTAC
SacI 1078 GAGCTC GAGCT^C
1092 GAGCTC
SacII 49 CCGCGG CCGC^GG
230 CCGCGG
SfaNI 923 GCATC GCATC(5/9)
1187 GCATC
Tru9I 1393 TTAA T^TAA
1433 TTAA
Van91I 744 CCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGGTCCTGG
XhoII 1167 GGATCT [GA]^GATC[CT]
1209 GGATCT
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII Sau3AI
Sau96I ScrFI SfcI SmaI SmlI
StyI TseI Tsp45I TspRI XmaCI
XmaI