Restriction map for
pFN21AE5736
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccII AccIII AflII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbvCI BfrI
BglI BlnI BlpI Bpu1102I BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I FauI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 1312 AACGTT AA^CGTT
AcyI 1493 GACGCC G[GA]^CG[CT]C
AlwNI 1568 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 997 ATTAAT AT^TAAT
AsnI 997 ATTAAT AT^TAAT
Asp700I 663 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 575 CCCGAG C^[CT]CG[GA]G
BamHI 1773 GGATCC G^GATCC
BanII 1941 GAGCTC G[GA]GC[CT]^C
BbrPI 1840 CACGTG CAC^GTG
BciVI 2342 GGATAC GTATCC(6/5)
BcnI 799 CCCGG CC^[GC]GG
BsaHI 1493 GACGCC G[GA]^CG[CT]C
BsaWI 1528 ACCGGA [AT]^CCGG[AT]
BsgI 1088 CTGCAC GTGCAG(16/14)
BsoBI 575 CCCGAG C^[CT]CG[GA]G
BspLU11I 1015 ACATGT A^CATGT
BspMI 2326 GCAGGT ACCTGC(4/8)
BsrDI 1757 GCAATG GCAATG(2/0)
Bst1107I 2369 GTATAC GTA^TAC
BstEII 2329 GGTCACC G^GT[ACGT]ACC
BstPI 2329 GGTCACC G^GT[ACGT]ACC
BstZ17I 2369 GTATAC GTA^TAC
DsaI 1166 CCATGG C^C[GA][CT]GG
EarI 1177 CTCTTC CTCTTC(1/4)
EcoNI 248 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2329 GGTCACC G^GT[ACGT]ACC
EcoRI 663 GAATTC G^AATTC
EcoT22I 911 ATGCAT ATGCA^T
Hin1I 1493 GACGCC G[GA]^CG[CT]C
HindIII 2130 AAGCTT A^AGCTT
Ksp632I 1177 CTCTTC CTCTTC(1/4)
MfeI 854 CAATTG C^AATTG
MunI 854 CAATTG C^AATTG
NciI 799 CCCGG CC^[GC]GG
NcoI 1166 CCATGG C^CATGG
NdeI 914 CATATG CA^TATG
NheI 1998 GCTAGC G^CTAGC
NsiI 911 ATGCAT ATGCA^T
NspI 1015 ACATGT [GA]CATG^[CT]
PflMI 1438 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1840 CACGTG CAC^GTG
PmlI 1840 CACGTG CAC^GTG
PshBI 997 ATTAAT AT^TAAT
Psp1406I 1312 AACGTT AA^CGTT
PstI 2292 CTGCAG CTGCA^G
PvuII 1571 CAGCTG CAG^CTG
SacI 1941 GAGCTC GAGCT^C
TaqI 366 TCGA T^CGA
TthHB8I 366 TCGA T^CGA
Van91I 1438 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 663 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 2184 GTCTAC GT^[AC][GT]AC
2369 GTATAC
AflIII 1015 ACATGT A^C[GA][CT]GT
1704 ACACGT
Alw44I 803 GTGCAC G^TGCAC
2042 GTGCAC
ApaLI 803 GTGCAC G^TGCAC
2042 GTGCAC
BcgI 408 GCAGAACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2085 GCACTTATTTCG
BfaI 1615 CTAG C^TAG
1999 CTAG
BglII 265 AGATCT A^GATCT
1853 AGATCT
BpmI 280 CTCCAG CTGGAG(16/14)
379 CTCCAG
BseRI 1244 CTCCTC GAGGAG(10/8)
2206 GAGGAG
DraII 1078 GGGACCC [GA]G^G[ACGT]CC[CT]
2097 GGGTCCC
EcoO109I 1078 GGGACCC [GA]G^G[ACGT]CC[CT]
2097 GGGTCCC
EcoRV 691 GATATC GAT^ATC
1321 GATATC
HgaI 298 GACGC GACGC(5/10)
1493 GACGC
HincII 820 GTTGAC GT[CT]^[GA]AC
1504 GTTGAC
HindII 820 GTTGAC GT[CT]^[GA]AC
1504 GTTGAC
MaeI 1615 CTAG C^TAG
1999 CTAG
MspA1I 967 CCGCTG C[AC]G^C[GT]G
1571 CAGCTG
PpuMI 1078 GGGACCC [GA]G^G[AT]CC[CT]
2097 GGGTCCC
SmlI 1938 CTTGAG C^T[CT][GA]AG
2465 CTCAAG
SspI 709 AATATT AAT^ATT
809 AATATT
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbsI
BbvI BclI BmyI BpuAI BsaAI
BsaBI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII