Restriction map for
pFN21AE2024
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BbrPI BcgI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT14I EcoT22I
FseI HindIII KpnI KspI MamI
MluI MroI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SphI SspBI
StyI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1281 CGCG CG^CG
AclI 222 AACGTT AA^CGTT
AflIII 1003 ACACGT A^C[GA][CT]GT
AhdI 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 966 CCTCAGG CC^T[ACGT]AGG
AspEI 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 963 GGACC G^G[AT]CC
AviII 595 TGCGCA TGC^GCA
BamHI 1175 GGATCC G^GATCC
BbeI 265 GGCGCC GGCGC^C
BbsI 135 GAAGAC GAAGAC(2/6)
BciVI 1315 GGATAC GTATCC(6/5)
BglII 1331 AGATCT A^GATCT
BpmI 731 CTCCAG CTGGAG(16/14)
BpuAI 135 GAAGAC GAAGAC(2/6)
BsaAI 1318 TACGTG [CT]AC^GT[GA]
BsaI 531 GAGACC GGTCTC(1/5)
BsiEI 1466 CGGTCG CG[GA][CT]^CG
BsrBI 1414 GAGCGG CCGCTC(-3/-3)
BssSI 237 CACGAG CACGAG(-5/-1)
BstUI 1281 CGCG CG^CG
BstXI 852 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 966 CCTCAGG CC^T[ACGT]AGG
DraII 962 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 966 CCTCAGG CC^T[ACGT]AGG
EcoO109I 962 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1291 GAATTC G^AATTC
EcoRV 3 GATATC GAT^ATC
FauI 1721 GCGGG CCCGC(4/6)
FspI 595 TGCGCA TGC^GCA
HpaI 225 GTTAAC GTT^AAC
KasI 265 GGCGCC G^GCGCC
MfeI 482 CAATTG C^AATTG
MslI 1645 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 482 CAATTG C^AATTG
MvnI 1281 CGCG CG^CG
NarI 265 GGCGCC GG^CGCC
PpuMI 962 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 222 AACGTT AA^CGTT
SbfI 1662 CCTGCAGG CCTGCA^GG
SfoI 265 GGCGCC GGC^GCC
SmaI 1776 CCCGGG CCC^GGG
SmlI 185 CTCAAG C^T[CT][GA]AG
SpeI 462 ACTAGT A^CTAGT
Sse8387I 1662 CCTGCAGG CCTGCA^GG
SspI 77 AATATT AAT^ATT
StuI 1303 AGGCCT AGG^CCT
TfiI 1528 GATTC G^A[AT]TC
XmaCI 1776 CCCGGG C^CCGGG
XmaI 1776 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 490 GAATTT [GA]^AATT[CT]
1291 GAATTC
AcyI 265 GGCGCC G[GA]^CG[CT]C
1192 GACGCC
AlwI 1176 GATCC GGATC(4/5)
1175 GGATC
AlwNI 1578 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1657 CAGACCCTG
ApoI 490 GAATTT [GA]^AATT[CT]
1291 GAATTC
AvaI 424 CCCGAG C^[CT]CG[GA]G
1776 CCCGGG
BalI 605 TGGCCA TGG^CCA
914 TGGCCA
BanI 265 GGCGCC G^G[CT][GA]CC
355 GGTGCC
BbvCI 889 CCTCAGC CCTCAGC(-5/-2)
1345 GCTGAGG
BclI 1023 TGATCA T^GATCA
1433 TGATCA
BsaHI 265 GGCGCC G[GA]^CG[CT]C
1192 GACGCC
BsoBI 424 CCCGAG C^[CT]CG[GA]G
1776 CCCGGG
BspMI 1573 ACCTGC ACCTGC(4/8)
1689 ACCTGC
BstYI 1175 GGATCC [GA]^GATC[CT]
1331 AGATCT
FbaI 1023 TGATCA T^GATCA
1433 TGATCA
HaeII 265 GGCGCC [GA]GCGC^[CT]
1370 AGCGCC
Hin1I 265 GGCGCC G[GA]^CG[CT]C
1192 GACGCC
MflI 1175 GGATCC [GA]^GATC[CT]
1331 AGATCT
MluNI 605 TGGCCA TGG^CCA
914 TGGCCA
MscI 605 TGGCCA TGG^CCA
914 TGGCCA
PflMI 277 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
346 CCAGCCTGTGG
PvuII 337 CAGCTG CAG^CTG
646 CAGCTG
SacI 191 GAGCTC GAGCT^C
583 GAGCTC
SfaNI 658 GATGC GCATC(5/9)
1284 GCATC
TaqI 701 TCGA T^CGA
945 TCGA
TthHB8I 701 TCGA T^CGA
945 TCGA
Van91I 277 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
346 CCAGCCTGTGG
XcmI 1324 CCAGAGGAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1641 CCATCACATCTGTGG
XhoII 1175 GGATCC [GA]^GATC[CT]
1331 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI XmnI