Restriction map for
pFN21AE5256
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI Eam1105I Eco47III
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
FseI HgaI HindIII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 166 CGCG CG^CG
AcyI 133 GGCGCC G[GA]^CG[CT]C
AlwI 76 GGATC GGATC(4/5)
AocI 529 CCTAAGG CC^T[ACGT]AGG
AseI 354 ATTAAT AT^TAAT
AsnI 354 ATTAAT AT^TAAT
Asp700I 378 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 551 CCCGGG C^[CT]CG[GA]G
AvaII 170 GGTCC G^G[AT]CC
AviII 2 TGCGCA TGC^GCA
BanI 133 GGCGCC G^G[CT][GA]CC
BbeI 133 GGCGCC GGCGC^C
BbsI 505 GAAGAC GAAGAC(2/6)
BclI 566 TGATCA T^GATCA
BglII 279 AGATCT A^GATCT
BpuAI 505 GAAGAC GAAGAC(2/6)
BsaHI 133 GGCGCC G[GA]^CG[CT]C
BsoBI 551 CCCGGG C^[CT]CG[GA]G
BspHI 662 TCATGA T^CATGA
BssSI 213 CTCGTG CACGAG(-5/-1)
BstUI 166 CGCG CG^CG
BstYI 279 AGATCT [GA]^GATC[CT]
Bsu36I 529 CCTAAGG CC^T[ACGT]AGG
Eco81I 529 CCTAAGG CC^T[ACGT]AGG
EcoT22I 380 ATGCAT ATGCA^T
FbaI 566 TGATCA T^GATCA
FspI 2 TGCGCA TGC^GCA
HaeII 133 GGCGCC [GA]GCGC^[CT]
Hin1I 133 GGCGCC G[GA]^CG[CT]C
HincII 325 GTCAAC GT[CT]^[GA]AC
HindII 325 GTCAAC GT[CT]^[GA]AC
KasI 133 GGCGCC G^GCGCC
MaeIII 361 GTCAC ^GT[ACGT]AC
MflI 279 AGATCT [GA]^GATC[CT]
MseI 355 TTAA T^TAA
MslI 630 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 166 CGCG CG^CG
NarI 133 GGCGCC GG^CGCC
NsiI 380 ATGCAT ATGCA^T
NspI 178 GCATGC [GA]CATG^[CT]
PshBI 354 ATTAAT AT^TAAT
RcaI 662 TCATGA T^CATGA
SapI 426 GCTCTTC GCTCTTC(1/4)
SfoI 133 GGCGCC GGC^GCC
SmaI 551 CCCGGG CCC^GGG
SphI 178 GCATGC GCATG^C
TfiI 659 GATTC G^A[AT]TC
Tru9I 355 TTAA T^TAA
Tsp45I 361 GTCAC ^GT[GC]AC
XhoII 279 AGATCT [GA]^GATC[CT]
XmaCI 551 CCCGGG C^CCGGG
XmaI 551 CCCGGG C^CCGGG
XmnI 378 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 391 GAATTC [GA]^AATT[CT]
406 GAATTC
ApoI 391 GAATTC [GA]^AATT[CT]
406 GAATTC
BanII 64 GGGCTC G[GA]GC[CT]^C
335 GGGCTC
BmyI 64 GGGCTC G[AGT]GC[ACT]^C
335 GGGCTC
BpmI 67 CTCCAG CTGGAG(16/14)
365 CTCCAG
BsiEI 26 CGGCCG CG[GA][CT]^CG
44 CGGCCG
BsiYI 151 CCCGGAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
152 CCGGAGCGGGG
BslI 151 CCCGGAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
152 CCGGAGCGGGG
BsmAI 644 GAGAC GTCTC(1/5)
218 GTCTC
BsmI 378 GAATGC GAATGC(1/-1)
382 GCATTC
Bsp1286I 64 GGGCTC G[AGT]GC[ACT]^C
335 GGGCTC
BsrBI 82 GAGCGG CCGCTC(-3/-3)
155 GAGCGG
BsrFI 24 GCCGGC [GA]^CCGG[CT]
46 GCCGGC
CfoI 3 GCGC GCG^C
134 GCGC
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
46 GCCGGC
Cfr13I 170 GGTCC G^G[ACGT]CC
248 GGCCC
EaeI 26 CGGCCG [CT]^GGCC[GA]
44 CGGCCG
EagI 26 CGGCCG C^GGCCG
44 CGGCCG
EarI 427 CTCTTC CTCTTC(1/4)
481 GAAGAG
EclXI 26 CGGCCG C^GGCCG
44 CGGCCG
Eco52I 26 CGGCCG C^GGCCG
44 CGGCCG
EcoRI 391 GAATTC G^AATTC
406 GAATTC
HhaI 3 GCGC GCG^C
134 GCGC
HinP1I 3 GCGC G^CGC
134 GCGC
HphI 537 GGTGA GGTGA(8/7)
555 GGTGA
Ksp632I 427 CTCTTC CTCTTC(1/4)
481 GAAGAG
NaeI 24 GCCGGC GCC^GGC
46 GCCGGC
NgoMIV 24 GCCGGC G^CCGGC
46 GCCGGC
Sau96I 170 GGTCC G^G[ACGT]CC
248 GGCCC
TaqI 605 TCGA T^CGA
657 TCGA
TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^
411 CAGTG
TthHB8I 605 TCGA T^CGA
657 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI SfaNI TseI
Tsp509I