##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccII          45  CGCG                 CG^CG
AccIII        347  TCCGGA               T^CCGGA
AciI           46  GCGG                 CCGC(-3/-1)
AcsI          454  GAATTT               [GA]^AATT[CT]
Alw44I        204  GTGCAC               G^TGCAC
ApaLI         204  GTGCAC               G^TGCAC
ApoI          454  GAATTT               [GA]^AATT[CT]
AspHI         204  GTGCAC               G[AT]GC[AT]^C
AvaII          48  GGTCC                G^G[AT]CC
BamHI           8  GGATCC               G^GATCC
BpmI          188  CTGGAG               CTGGAG(16/14)
BsaWI         347  TCCGGA               [AT]^CCGG[AT]
BseAI         347  TCCGGA               T^CCGGA
BseRI         151  GAGGAG               GAGGAG(10/8)
BsgI          300  CTGCAC               GTGCAG(16/14)
BsiHKAI       204  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      262  TGTACA               T^GTACA
BspEI         347  TCCGGA               T^CCGGA
BspMI         386  GCAGGT               ACCTGC(4/8)
BsrGI         262  TGTACA               T^GTACA
BstF5I        432  GGATG                GGATG(2/0)
BstUI          45  CGCG                 CG^CG
DraIII        318  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRV         436  GATATC               GAT^ATC
FokI          432  GGATG                GGATG(9/13)
MaeII         424  ACGT                 A^CGT
MaeIII        197  GTAAC                ^GT[ACGT]AC
MroI          347  TCCGGA               T^CCGGA
MvnI           45  CGCG                 CG^CG
SspBI         262  TGTACA               T^GTACA
XcmI          464  CCAATGCCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          263  GTAC                 GT^AC
              422  GTAC                
BfaI           64  CTAG                 C^TAG
              395  CTAG                
BmyI          204  GTGCAC               G[AGT]GC[ACT]^C
              390  GTGCCC              
Bsp1286I      204  GTGCAC               G[AGT]GC[ACT]^C
              390  GTGCCC              
BstNI         428  CCTGG                CC^[AT]GG
              559  CCAGG               
BstYI           8  GGATCC               [GA]^GATC[CT]
              166  GGATCT              
EcoRII        428  CCTGG                ^CC[AT]GG
              559  CCAGG               
HaeIII          3  GGCC                 GG^CC
              117  GGCC                
MaeI           64  CTAG                 C^TAG
              395  CTAG                
MflI            8  GGATCC               [GA]^GATC[CT]
              166  GGATCT              
MvaI          428  CCTGG                CC^[AT]GG
              559  CCAGG               
RsaI          263  GTAC                 GT^AC
              422  GTAC                
XhoII           8  GGATCC               [GA]^GATC[CT]
              166  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AluI        AlwI        AlwNI       AvaI        BanI        
BbvI        BcnI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       BsrI        BssKI       
Cfr13I      DpnII       EarI        Fnu4HI      HapII       
HinfI       HpaII       ItaI        Ksp632I     MboI        
MboII       MnlI        MspA1I      MspI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PvuII       Sau3AI      Sau96I      ScrFI       SmaI        
TseI        Tsp509I     TspRI       XmaCI       XmaI