Restriction map for
pFN21AB6147
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BamHI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NruI
NsiI NspI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1486 GACGTC GACGT^C
AgeI 104 ACCGGT A^CCGGT
AhdI 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1233 GATCC GGATC(4/5)
AspEI 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1652 TGGCCA TGG^CCA
BbrPI 1703 CACGTG CAC^GTG
BcgI 351 CGAGAAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1444 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1169 AGATCT A^GATCT
BspHI 1055 TCATGA T^CATGA
BsrFI 104 ACCGGT [GA]^CCGG[CT]
BsrI 1140 CCAGT ACTGG(1/-1)
BstXI 1325 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 104 ACCGGT [GA]^CCGG[CT]
DrdI 580 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 966 GAAGAG CTCTTC(1/4)
HgaI 8 GCGTC GACGC(5/10)
HincII 1735 GTTGAC GT[CT]^[GA]AC
HindII 1735 GTTGAC GT[CT]^[GA]AC
Ksp632I 966 GAAGAG CTCTTC(1/4)
KspI 895 CCGCGG CCGC^GG
MluNI 1652 TGGCCA TGG^CCA
MscI 1652 TGGCCA TGG^CCA
MslI 591 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1225 GCTAGC G^CTAGC
NotI 891 GCGGCCGC GC^GGCCGC
PaeR7I 1045 CTCGAG C^TCGAG
PinAI 104 ACCGGT A^CCGGT
PmaCI 1703 CACGTG CAC^GTG
PmlI 1703 CACGTG CAC^GTG
PshAI 145 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1055 TCATGA T^CATGA
SacI 1042 GAGCTC GAGCT^C
SacII 895 CCGCGG CCGC^GG
SapI 966 GAAGAGC GCTCTTC(1/4)
SbfI 996 CCTGCAGG CCTGCA^GG
SfiI 1443 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 221 CCCGGG CCC^GGG
Sse8387I 996 CCTGCAGG CCTGCA^GG
StuI 956 AGGCCT AGG^CCT
TfiI 1617 GATTC G^A[AT]TC
XcmI 164 CCAGCTCCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1045 CTCGAG C^TCGAG
XmaCI 221 CCCGGG C^CCGGG
XmaI 221 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 498 GTGCTC G[AT]GC[AT]^C
1042 GAGCTC
AviII 74 TGCGCA TGC^GCA
1319 TGCGCA
BanI 823 GGCGCC G^G[CT][GA]CC
1312 GGCGCC
BbeI 823 GGCGCC GGCGC^C
1312 GGCGCC
BbsI 187 GAAGAC GAAGAC(2/6)
1107 GAAGAC
BbvCI 123 GCTGAGG CCTCAGC(-5/-2)
1290 GCTGAGG
BfaI 337 CTAG C^TAG
1226 CTAG
BpuAI 187 GAAGAC GAAGAC(2/6)
1107 GAAGAC
BsaAI 1648 TACGTG [CT]AC^GT[GA]
1703 CACGTG
BsaWI 104 ACCGGT [AT]^CCGG[AT]
148 TCCGGT
BsiEI 522 CGGCCG CG[GA][CT]^CG
892 CGGCCG
BsiHKAI 498 GTGCTC G[AT]GC[AT]^C
1042 GAGCTC
BssHII 975 GCGCGC G^CGCGC
977 GCGCGC
BssSI 747 CTCGTG CACGAG(-5/-1)
1588 CTCGTG
BstYI 1169 AGATCT [GA]^GATC[CT]
1232 AGATCC
EagI 522 CGGCCG C^GGCCG
892 CGGCCG
EclXI 522 CGGCCG C^GGCCG
892 CGGCCG
Eco52I 522 CGGCCG C^GGCCG
892 CGGCCG
FspI 74 TGCGCA TGC^GCA
1319 TGCGCA
KasI 823 GGCGCC G^GCGCC
1312 GGCGCC
MaeI 337 CTAG C^TAG
1226 CTAG
MaeIII 152 GTCAC ^GT[ACGT]AC
589 GTCAC
MflI 1169 AGATCT [GA]^GATC[CT]
1232 AGATCC
NarI 823 GGCGCC GG^CGCC
1312 GGCGCC
SfaNI 647 GCATC GCATC(5/9)
785 GCATC
SfoI 823 GGCGCC GGC^GCC
1312 GGCGCC
TaqI 724 TCGA T^CGA
1046 TCGA
Tsp45I 152 GTCAC ^GT[GC]AC
589 GTCAC
TthHB8I 724 TCGA T^CGA
1046 TCGA
XhoII 1169 AGATCT [GA]^GATC[CT]
1232 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TseI TspRI Van91I