##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstZ17I     CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FokI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboII       MluI        
MroI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcsI          161  AAATTC               [GA]^AATT[CT]
AocI           56  CCTCAGG              CC^T[ACGT]AGG
ApoI          161  AAATTC               [GA]^AATT[CT]
BalI           84  TGGCCA               TGG^CCA
BanI          169  GGCACC               G^G[CT][GA]CC
BbvI          197  GCTGC                GCAGC(8/12)
BclI           96  TGATCA               T^GATCA
BglII          91  AGATCT               A^GATCT
BsaJI          81  CCTTGG               C^C[ACGT][ACGT]GG
BsmAI         131  GTCTC                GTCTC(1/5)
BsrDI         126  GCAATG               GCAATG(2/0)
BstYI          91  AGATCT               [GA]^GATC[CT]
Bsu36I         56  CCTCAGG              CC^T[ACGT]AGG
Cac8I         142  GCCTGC               GC[ACGT]^[ACGT]GC
EaeI           84  TGGCCA               [CT]^GGCC[GA]
Eco81I         56  CCTCAGG              CC^T[ACGT]AGG
EcoT14I        81  CCTTGG               C^C[AT][AT]GG
FbaI           96  TGATCA               T^GATCA
Fnu4HI        197  GCTGC                GC^[ACGT]GC
HgaI          110  GCGTC                GACGC(5/10)
ItaI          197  GCTGC                GC^[ACGT]GC
MfeI          105  CAATTG               C^AATTG
MflI           91  AGATCT               [GA]^GATC[CT]
MluNI          84  TGGCCA               TGG^CCA
MscI           84  TGGCCA               TGG^CCA
MunI          105  CAATTG               C^AATTG
NlaIV         169  GGCACC               GG[ACGT]^[ACGT]CC
StuI           78  AGGCCT               AGG^CCT
StyI           81  CCTTGG               C^C[AT][AT]GG
TseI          197  GCTGC                G^C[AT]GC
TspRI         117  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII          91  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

DdeI           47  CTGAG                C^T[ACGT]AG
               57  CTCAG               
MaeIII         65  GTAAC                ^GT[ACGT]AC
              176  GTTAC               
Tsp509I       106  AATT                 ^AATT
              162  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

DpnII       HaeIII      MboI        MnlI        NdeII       
Sau3AI