Restriction map for
pFN21AE5812
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# NO CUT ENZYMES ( 65 enzymes) #
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AccI AclI AcsI AflII AgeI
ApoI AscI AseI AsnI AspI
AvrII BclI BfrI BlnI BsaBI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV EcoT22I FbaI HindIII
HpaI KspI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 3430 GACGTC GACGT^C
Acc65I 2201 GGTACC G^GTACC
AccIII 1625 TCCGGA T^CCGGA
AflIII 3329 ACATGT A^C[GA][CT]GT
Aor51HI 698 AGCGCT AGC^GCT
Asp718I 2201 GGTACC G^GTACC
AviII 3491 TGCGCA TGC^GCA
BalI 1496 TGGCCA TGG^CCA
BbeI 2463 GGCGCC GGCGC^C
BcgI 1076 GCACTGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1155 GGATAC GTATCC(6/5)
BfaI 1234 CTAG C^TAG
BglII 1511 AGATCT A^GATCT
BseAI 1625 TCCGGA T^CCGGA
BsmBI 1690 GAGACG CGTCTC(1/5)
BsmI 821 GCATTC GAATGC(1/-1)
BspEI 1625 TCCGGA T^CCGGA
BspHI 1280 TCATGA T^CATGA
BspLU11I 3329 ACATGT A^CATGT
BsrDI 3051 CATTGC GCAATG(2/0)
BstEII 1707 GGTAACC G^GT[ACGT]ACC
BstPI 1707 GGTAACC G^GT[ACGT]ACC
DraI 1486 TTTAAA TTT^AAA
DraIII 3301 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1164 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 698 AGCGCT AGC^GCT
EcoO65I 1707 GGTAACC G^GT[ACGT]ACC
FseI 331 GGCCGGCC GGCCGG^CC
FspI 3491 TGCGCA TGC^GCA
HincII 1393 GTCAAC GT[CT]^[GA]AC
HindII 1393 GTCAAC GT[CT]^[GA]AC
KasI 2463 GGCGCC G^GCGCC
KpnI 2201 GGTACC GGTAC^C
MaeI 1234 CTAG C^TAG
MluNI 1496 TGGCCA TGG^CCA
MroI 1625 TCCGGA T^CCGGA
MscI 1496 TGGCCA TGG^CCA
NarI 2463 GGCGCC GG^CGCC
NcoI 3353 CCATGG C^CATGG
NheI 1233 GCTAGC G^CTAGC
PaeR7I 3316 CTCGAG C^TCGAG
PflMI 3040 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1280 TCATGA T^CATGA
ScaI 398 AGTACT AGT^ACT
SfoI 2463 GGCGCC GGC^GCC
SgrAI 2459 CGCCGGCG C[GA]^CCGG[CT]G
Tsp509I 1026 AATT ^AATT
Van91I 3040 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3316 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 2463 GGCGCC G[GA]^CG[CT]C
3430 GACGTC
AhdI 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1669 GACGTGGTGTC
AocI 1932 CCTCAGG CC^T[ACGT]AGG
3273 CCTGAGG
Asp700I 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2709 GAAGTGGTTC
AspEI 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1669 GACGTGGTGTC
BbrPI 2254 CACGTG CAC^GTG
2931 CACGTG
BbsI 1479 GAAGAC GAAGAC(2/6)
3469 GAAGAC
BpuAI 1479 GAAGAC GAAGAC(2/6)
3469 GAAGAC
BsaHI 2463 GGCGCC G[GA]^CG[CT]C
3430 GACGTC
BsaWI 1625 TCCGGA [AT]^CCGG[AT]
2353 ACCGGA
Bsu36I 1932 CCTCAGG CC^T[ACGT]AGG
3273 CCTGAGG
EagI 1584 CGGCCG C^GGCCG
1804 CGGCCG
Eam1105I 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1669 GACGTGGTGTC
EclXI 1584 CGGCCG C^GGCCG
1804 CGGCCG
Eco52I 1584 CGGCCG C^GGCCG
1804 CGGCCG
Eco81I 1932 CCTCAGG CC^T[ACGT]AGG
3273 CCTGAGG
Hin1I 2463 GGCGCC G[GA]^CG[CT]C
3430 GACGTC
MseI 413 TTAA T^TAA
1487 TTAA
NspI 1013 ACATGC [GA]CATG^[CT]
3329 ACATGT
PmaCI 2254 CACGTG CAC^GTG
2931 CACGTG
PmlI 2254 CACGTG CAC^GTG
2931 CACGTG
SacI 165 GAGCTC GAGCT^C
814 GAGCTC
SapI 12 GAAGAGC GCTCTTC(1/4)
2644 GAAGAGC
StuI 527 AGGCCT AGG^CCT
2078 AGGCCT
TfiI 725 GAATC G^A[AT]TC
3068 GATTC
Tru9I 413 TTAA T^TAA
1487 TTAA
XcmI 1010 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2468 CCACTGAGCCCGTGG
XmnI 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2709 GAAGTGGTTC
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI