Restriction map for
pFN21AE5324
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BglII BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FauI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 585 GTATAC GT^[AC][GT]AC
AfaI 498 GTAC GT^AC
AflII 195 CTTAAG C^TTAAG
Aor51HI 7 AGCGCT AGC^GCT
ApaI 891 GGGCCC GGGCC^C
AspHI 875 GTGCTC G[AT]GC[AT]^C
BalI 707 TGGCCA TGG^CCA
BanI 383 GGTGCC G^G[CT][GA]CC
BanII 891 GGGCCC G[GA]GC[CT]^C
BciVI 477 GTATCC GTATCC(6/5)
BfrI 195 CTTAAG C^TTAAG
BlpI 3 GCTGAGC GC^T[ACGT]AGC
BpmI 87 CTGGAG CTGGAG(16/14)
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
BsiEI 330 CGATCG CG[GA][CT]^CG
BsiHKAI 875 GTGCTC G[AT]GC[AT]^C
BsmI 470 GCATTC GAATGC(1/-1)
Bst1107I 585 GTATAC GTA^TAC
BstXI 612 CCATTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 854 AGATCC [GA]^GATC[CT]
BstZ17I 585 GTATAC GTA^TAC
CelII 3 GCTGAGC GC^T[ACGT]AGC
EaeI 707 TGGCCA [CT]^GGCC[GA]
EarI 797 CTCTTC CTCTTC(1/4)
Eco47III 7 AGCGCT AGC^GCT
EcoNI 451 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 506 ATGCAT ATGCA^T
HaeII 7 AGCGCT [GA]GCGC^[CT]
HindIII 198 AAGCTT A^AGCTT
Ksp632I 797 CTCTTC CTCTTC(1/4)
MaeIII 414 GTTAC ^GT[ACGT]AC
MflI 854 AGATCC [GA]^GATC[CT]
MluNI 707 TGGCCA TGG^CCA
MscI 707 TGGCCA TGG^CCA
MspA1I 617 CAGCTG C[AC]G^C[GT]G
NsiI 506 ATGCAT ATGCA^T
PflMI 272 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 375 GAGTC GAGTC(4/5)
PpuMI 254 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 172 CTGCAG CTGCA^G
PvuI 330 CGATCG CGAT^CG
PvuII 617 CAGCTG CAG^CTG
RsaI 498 GTAC GT^AC
SexAI 692 ACCTGGT A^CC[AT]GGT
SfcI 172 CTGCAG C^T[GA][CT]AG
SmlI 195 CTTAAG C^T[CT][GA]AG
TaqI 329 TCGA T^CGA
TfiI 825 GATTC G^A[AT]TC
TthHB8I 329 TCGA T^CGA
Van91I 272 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 854 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 46 GAATTT [GA]^AATT[CT]
112 AAATTC
AlwI 855 GATCC GGATC(4/5)
104 GGATC
AlwNI 175 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
496 CAGTACCTG
ApoI 46 GAATTT [GA]^AATT[CT]
112 AAATTC
AvrII 15 CCTAGG C^CTAGG
829 CCTAGG
BclI 182 TGATCA T^GATCA
815 TGATCA
BcnI 336 CCCGG CC^[GC]GG
636 CCGGG
BfaI 16 CTAG C^TAG
830 CTAG
BglI 149 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
386 GCCTTCTGGGC
BlnI 15 CCTAGG C^CTAGG
829 CCTAGG
BmyI 875 GTGCTC G[AGT]GC[ACT]^C
891 GGGCCC
Bsp1286I 875 GTGCTC G[AGT]GC[ACT]^C
891 GGGCCC
BsrI 119 ACTGG ACTGG(1/-1)
755 CCAGT
CfoI 8 GCGC GCG^C
658 GCGC
FbaI 182 TGATCA T^GATCA
815 TGATCA
HapII 337 CCGG C^CGG
636 CCGG
HhaI 8 GCGC GCG^C
658 GCGC
HinP1I 8 GCGC G^CGC
658 GCGC
HinfI 375 GAGTC G^A[ACGT]TC
825 GATTC
HpaII 337 CCGG C^CGG
636 CCGG
MaeI 16 CTAG C^TAG
830 CTAG
MspI 337 CCGG C^CGG
636 CCGG
NciI 336 CCCGG CC^[GC]GG
636 CCGGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AvaII BbsI BbvI BpuAI
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HphI ItaI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp509I TspRI