Restriction map for
pFN21AE1969
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AviII BbeI BbsI
BcgI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FspI HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1530 GGTACC G^GTACC
AccI 1114 GTAGAC GT^[AC][GT]AC
AcsI 655 GAATTC [GA]^AATT[CT]
AcyI 336 GGCGTC G[GA]^CG[CT]C
AlwNI 1283 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 616 CCTGAGG CC^T[ACGT]AGG
ApoI 655 GAATTC [GA]^AATT[CT]
Asp718I 1530 GGTACC G^GTACC
AspI 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1560 CCTAGG C^CTAGG
BamHI 621 GGATCC G^GATCC
BanII 208 GAGCTC G[GA]GC[CT]^C
BbrPI 1486 CACGTG CAC^GTG
BciVI 1594 GTATCC GTATCC(6/5)
BclI 287 TGATCA T^GATCA
BlnI 1560 CCTAGG C^CTAGG
BpmI 118 CTGGAG CTGGAG(16/14)
BsaHI 336 GGCGTC G[GA]^CG[CT]C
BsaWI 1502 TCCGGT [AT]^CCGG[AT]
BseRI 915 CTCCTC GAGGAG(10/8)
BsmI 106 GCATTC GAATGC(1/-1)
BstBI 652 TTCGAA TT^CGAA
BstYI 621 GGATCC [GA]^GATC[CT]
Bsu36I 616 CCTGAGG CC^T[ACGT]AGG
DraI 498 TTTAAA TTT^AAA
DraII 611 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 616 CCTGAGG CC^T[ACGT]AGG
EcoO109I 611 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 655 GAATTC G^AATTC
FbaI 287 TGATCA T^GATCA
FseI 1422 GGCCGGCC GGCCGG^CC
HaeII 1285 GGCGCT [GA]GCGC^[CT]
Hin1I 336 GGCGTC G[GA]^CG[CT]C
HincII 1473 GTTGAC GT[CT]^[GA]AC
HindII 1473 GTTGAC GT[CT]^[GA]AC
HindIII 1099 AAGCTT A^AGCTT
KpnI 1530 GGTACC GGTAC^C
MflI 621 GGATCC [GA]^GATC[CT]
MspA1I 1534 CCGCTG C[AC]G^C[GT]G
NaeI 1423 GCCGGC GCC^GGC
NgoMIV 1423 GCCGGC G^CCGGC
NspV 652 TTCGAA TT^CGAA
PaeR7I 1159 CTCGAG C^TCGAG
PflFI 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1567 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 601 GACTC GAGTC(4/5)
PmaCI 1486 CACGTG CAC^GTG
PmlI 1486 CACGTG CAC^GTG
SacI 208 GAGCTC GAGCT^C
SfuI 652 TTCGAA TT^CGAA
Tth111I 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1567 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1159 CTCGAG C^TCGAG
XhoII 621 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 622 GATCC GGATC(4/5)
621 GGATC
AspHI 208 GAGCTC G[AT]GC[AT]^C
1330 GAGCAC
AvaI 1159 CTCGAG C^[CT]CG[GA]G
1441 CTCGGG
BbvCI 1203 GCTGAGG CCTCAGC(-5/-2)
1375 GCTGAGG
BsaI 1348 GAGACC GGTCTC(1/5)
1522 GAGACC
BsgI 1315 CTGCAC GTGCAG(16/14)
1492 CTGCAC
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
1330 GAGCAC
BsoBI 1159 CTCGAG C^[CT]CG[GA]G
1441 CTCGGG
BspMI 1409 ACCTGC ACCTGC(4/8)
294 GCAGGT
BsrFI 1394 ACCGGC [GA]^CCGG[CT]
1423 GCCGGC
CfoI 1272 GCGC GCG^C
1286 GCGC
Cfr10I 1394 ACCGGC [GA]^CCGG[CT]
1423 GCCGGC
EarI 400 GAAGAG CTCTTC(1/4)
817 GAAGAG
HhaI 1272 GCGC GCG^C
1286 GCGC
HinP1I 1272 GCGC G^CGC
1286 GCGC
Ksp632I 400 GAAGAG CTCTTC(1/4)
817 GAAGAG
NcoI 44 CCATGG C^CATGG
875 CCATGG
PstI 1152 CTGCAG CTGCA^G
1537 CTGCAG
SfcI 1152 CTGCAG C^T[GA][CT]AG
1537 CTGCAG
TaqI 653 TCGA T^CGA
1160 TCGA
Tsp45I 49 GTGAC ^GT[GC]AC
386 GTGAC
TthHB8I 653 TCGA T^CGA
1160 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BanI BbvI BcnI BfaI BmyI
BsaAI BsaJI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StuI
StyI TfiI Tru9I TseI Tsp509I
TspRI XcmI