Restriction map for
pFN21AE6763
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BcgI BfrI BlnI BsaI
BseAI BsiEI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
Tth111I Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 2306 CGCG CG^CG
ApaI 2234 GGGCCC GGGCC^C
AvaI 241 CTCGAG C^[CT]CG[GA]G
BalI 2085 TGGCCA TGG^CCA
BciVI 1762 GGATAC GTATCC(6/5)
BglI 2078 GCCGTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 79 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC
BsaBI 1740 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 499 ACCGGA [AT]^CCGG[AT]
BsoBI 241 CTCGAG C^[CT]CG[GA]G
BspMI 2222 ACCTGC ACCTGC(4/8)
BsrFI 520 GCCGGT [GA]^CCGG[CT]
BstBI 14 TTCGAA TT^CGAA
BstUI 2306 CGCG CG^CG
CelII 79 GCTGAGC GC^T[ACGT]AGC
Cfr10I 520 GCCGGT [GA]^CCGG[CT]
DrdI 2152 GACGTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2085 TGGCCA [CT]^GGCC[GA]
EcoNI 849 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 261 CCCGC CCCGC(4/6)
HindIII 357 AAGCTT A^AGCTT
MamI 1740 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2085 TGGCCA TGG^CCA
MscI 2085 TGGCCA TGG^CCA
MvnI 2306 CGCG CG^CG
NspV 14 TTCGAA TT^CGAA
PaeR7I 241 CTCGAG C^TCGAG
PshAI 1231 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1433 GCTCTTC GCTCTTC(1/4)
ScaI 2186 AGTACT AGT^ACT
SexAI 1766 ACCTGGT A^CC[AT]GGT
SfuI 14 TTCGAA TT^CGAA
SmlI 241 CTCGAG C^T[CT][GA]AG
SspI 1132 AATATT AAT^ATT
StuI 344 AGGCCT AGG^CCT
SwaI 116 ATTTAAAT ATTT^AAAT
XbaI 297 TCTAGA T^CTAGA
XhoI 241 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 736 GTCTAC GT^[AC][GT]AC
913 GTCTAC
AfaI 591 GTAC GT^AC
2187 GTAC
AlwNI 482 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
844 CAGCGCCTG
AvaII 1103 GGACC G^G[AT]CC
1515 GGACC
BamHI 627 GGATCC G^GATCC
2024 GGATCC
BbsI 205 GAAGAC GAAGAC(2/6)
1305 GAAGAC
BbvCI 1671 CCTCAGC CCTCAGC(-5/-2)
2238 CCTCAGC
BcnI 981 CCCGG CC^[GC]GG
1164 CCGGG
BglII 710 AGATCT A^GATCT
971 AGATCT
BpuAI 205 GAAGAC GAAGAC(2/6)
1305 GAAGAC
BsaAI 998 CACGTA [CT]AC^GT[GA]
1810 CACGTA
BspHI 1058 TCATGA T^CATGA
1775 TCATGA
BspLU11I 929 ACATGT A^CATGT
1853 ACATGT
BsrBI 262 CCGCTC CCGCTC(-3/-3)
556 GAGCGG
BstAPI 481 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2409 GCACGCCCTGC
DraI 117 TTTAAA TTT^AAA
2488 TTTAAA
DraII 2234 GGGCCCT [GA]G^G[ACGT]CC[CT]
2390 AGGGCCT
EcoO109I 2234 GGGCCCT [GA]G^G[ACGT]CC[CT]
2390 AGGGCCT
HaeII 845 AGCGCC [GA]GCGC^[CT]
1343 GGCGCT
MslI 888 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1848 CATCAACATG
NciI 981 CCCGG CC^[GC]GG
1164 CCGGG
NcoI 1046 CCATGG C^CATGG
2127 CCATGG
NspI 929 ACATGT [GA]CATG^[CT]
1853 ACATGT
PleI 1876 GACTC GAGTC(4/5)
832 GAGTC
RcaI 1058 TCATGA T^CATGA
1775 TCATGA
RsaI 591 GTAC GT^AC
2187 GTAC
SacI 82 GAGCTC GAGCT^C
91 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AatII AciI AcsI AcyI AflIII
AluI AlwI ApoI Asp700I AspHI
BanII BbvI BclI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI