Restriction map for
pFN21AE1663
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI MwoI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TspRI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 478 GGTACC G^GTACC
AlwNI 248 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 271 ATTAAT AT^TAAT
AsnI 271 ATTAAT AT^TAAT
Asp718I 478 GGTACC G^GTACC
AspHI 501 GAGCTC G[AT]GC[AT]^C
AspI 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 478 GGTACC G^G[CT][GA]CC
BanII 501 GAGCTC G[GA]GC[CT]^C
BfaI 262 CTAG C^TAG
BmyI 501 GAGCTC G[AGT]GC[ACT]^C
BseRI 451 GAGGAG GAGGAG(10/8)
BsiHKAI 501 GAGCTC G[AT]GC[AT]^C
BsmFI 485 GGGAC GGGAC(10/14)
Bsp1286I 501 GAGCTC G[AGT]GC[ACT]^C
BspHI 209 TCATGA T^CATGA
BsrI 11 CCAGT ACTGG(1/-1)
BstYI 357 AGATCC [GA]^GATC[CT]
CfoI 457 GCGC GCG^C
DdeI 329 CTCAG C^T[ACGT]AG
DsaI 446 CCACGG C^C[GA][CT]GG
EarI 472 GAAGAG CTCTTC(1/4)
HaeII 456 GGCGCT [GA]GCGC^[CT]
HhaI 457 GCGC GCG^C
HinP1I 457 GCGC G^CGC
HphI 370 GGTGA GGTGA(8/7)
KpnI 478 GGTACC GGTAC^C
Ksp632I 472 GAAGAG CTCTTC(1/4)
MaeI 262 CTAG C^TAG
MaeII 41 ACGT A^CGT
MflI 357 AGATCC [GA]^GATC[CT]
MslI 36 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 134 CATATG CA^TATG
NlaIV 478 GGTACC GG[ACGT]^[ACGT]CC
PflFI 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 534 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 316 GAGTC GAGTC(4/5)
PshBI 271 ATTAAT AT^TAAT
PstI 377 CTGCAG CTGCA^G
RcaI 209 TCATGA T^CATGA
SacI 501 GAGCTC GAGCT^C
TaqI 56 TCGA T^CGA
TfiI 206 GATTC G^A[AT]TC
Tsp45I 371 GTGAC ^GT[GC]AC
Tth111I 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 56 TCGA T^CGA
Van91I 534 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 261 TCTAGA T^CTAGA
XhoII 357 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 27 GCGG CCGC(-3/-1)
143 GCGG
AcsI 97 GAATTT [GA]^AATT[CT]
218 AAATTT
AlwI 78 GATCC GGATC(4/5)
358 GATCC
ApoI 97 GAATTT [GA]^AATT[CT]
218 AAATTT
AvaII 118 GGACC G^G[AT]CC
165 GGACC
BbvI 379 GCAGC GCAGC(8/12)
376 GCTGC
BcnI 16 CCGGG CC^[GC]GG
482 CCGGG
BstNI 81 CCTGG CC^[AT]GG
534 CCAGG
EcoRII 81 CCTGG ^CC[AT]GG
534 CCAGG
Fnu4HI 376 GCTGC GC^[ACGT]GC
379 GCAGC
HaeIII 339 GGCC GG^CC
531 GGCC
HapII 16 CCGG C^CGG
482 CCGG
HgaI 373 GACGC GACGC(5/10)
244 GCGTC
HinfI 206 GATTC G^A[ACGT]TC
316 GAGTC
HpaII 16 CCGG C^CGG
482 CCGG
ItaI 376 GCTGC GC^[ACGT]GC
379 GCAGC
MaeIII 73 GTTAC ^GT[ACGT]AC
371 GTGAC
MspI 16 CCGG C^CGG
482 CCGG
MvaI 81 CCTGG CC^[AT]GG
534 CCAGG
NciI 16 CCGGG CC^[GC]GG
482 CCGGG
SfcI 200 CTATAG C^T[GA][CT]AG
377 CTGCAG
TseI 376 GCTGC G^C[AT]GC
379 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BssKI BstF5I Cfr13I DpnII FokI
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
Tru9I Tsp509I