Restriction map for
pFN21AE9524
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BclI BcnI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmFI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 1023 GTCTAC GT^[AC][GT]AC
AccII 795 CGCG CG^CG
AcyI 487 GGCGTC G[GA]^CG[CT]C
AflIII 224 ACGTGT A^C[GA][CT]GT
AlwNI 664 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 529 CCTGAGG CC^T[ACGT]AGG
Aor51HI 392 AGCGCT AGC^GCT
ApaI 674 GGGCCC GGGCC^C
BalI 170 TGGCCA TGG^CCA
BamHI 766 GGATCC G^GATCC
BanI 574 GGCACC G^G[CT][GA]CC
BanII 674 GGGCCC G[GA]GC[CT]^C
BbvCI 669 GCTGAGG CCTCAGC(-5/-2)
BciVI 292 GGATAC GTATCC(6/5)
BglI 376 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 694 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 694 GCTCAGC GC^T[ACGT]AGC
BsaHI 487 GGCGTC G[GA]^CG[CT]C
BseRI 244 CTCCTC GAGGAG(10/8)
BsiEI 306 CGGTCG CG[GA][CT]^CG
BsmBI 489 CGTCTC CGTCTC(1/5)
Bsp1407I 809 TGTACA T^GTACA
BspMI 349 GCAGGT ACCTGC(4/8)
BsrDI 81 CATTGC GCAATG(2/0)
BsrFI 518 GCCGGT [GA]^CCGG[CT]
BsrGI 809 TGTACA T^GTACA
BstUI 795 CGCG CG^CG
BstYI 766 GGATCC [GA]^GATC[CT]
Bsu36I 529 CCTGAGG CC^T[ACGT]AGG
CelII 694 GCTCAGC GC^T[ACGT]AGC
Cfr10I 518 GCCGGT [GA]^CCGG[CT]
DpnII 767 GATC ^GATC
EaeI 170 TGGCCA [CT]^GGCC[GA]
Eco47III 392 AGCGCT AGC^GCT
Eco81I 529 CCTGAGG CC^T[ACGT]AGG
HaeII 392 AGCGCT [GA]GCGC^[CT]
HapII 519 CCGG C^CGG
HgaI 488 GCGTC GACGC(5/10)
Hin1I 487 GGCGTC G[GA]^CG[CT]C
HindIII 932 AAGCTT A^AGCTT
HpaII 519 CCGG C^CGG
MboI 767 GATC ^GATC
MflI 766 GGATCC [GA]^GATC[CT]
MluNI 170 TGGCCA TGG^CCA
MscI 170 TGGCCA TGG^CCA
MspI 519 CCGG C^CGG
MvnI 795 CGCG CG^CG
NdeII 767 GATC ^GATC
PflMI 831 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 453 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 538 CTGCAG CTGCA^G
Sau3AI 767 GATC ^GATC
SfiI 375 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 444 GCATGC GCATG^C
SspBI 809 TGTACA T^GTACA
TfiI 976 GAATC G^A[AT]TC
Van91I 831 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 158 CCATGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 766 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 767 GATCC GGATC(4/5)
766 GGATC
AvaII 459 GGTCC G^G[AT]CC
473 GGACC
BpmI 106 CTCCAG CTGGAG(16/14)
513 CTGGAG
BsaAI 600 TACGTG [CT]AC^GT[GA]
784 TACGTG
BsmAI 490 GTCTC GTCTC(1/5)
1018 GTCTC
BspHI 914 TCATGA T^CATGA
941 TCATGA
BsrI 372 ACTGG ACTGG(1/-1)
560 ACTGG
BstXI 158 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCATGTACATGG
CfoI 180 GCGC GCG^C
393 GCGC
EcoT14I 158 CCATGG C^C[AT][AT]GG
831 CCATGG
HhaI 180 GCGC GCG^C
393 GCGC
HinP1I 180 GCGC G^CGC
393 GCGC
HincII 220 GTCAAC GT[CT]^[GA]AC
817 GTCAAC
HindII 220 GTCAAC GT[CT]^[GA]AC
817 GTCAAC
HphI 738 GGTGA GGTGA(8/7)
854 TCACC
MspA1I 282 CAGCTG C[AC]G^C[GT]G
664 CAGCTG
NcoI 158 CCATGG C^CATGG
831 CCATGG
NspI 444 GCATGC [GA]CATG^[CT]
985 ACATGC
PleI 592 GAGTC GAGTC(4/5)
706 GAGTC
PvuII 282 CAGCTG CAG^CTG
664 CAGCTG
RcaI 914 TCATGA T^CATGA
941 TCATGA
SfcI 344 CTGTAG C^T[GA][CT]AG
538 CTGCAG
StyI 158 CCATGG C^C[AT][AT]GG
831 CCATGG
Tsp45I 369 GTCAC ^GT[GC]AC
853 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII DsaI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeII MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI TseI TspRI