##################################
# NO CUT ENZYMES (  223 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AfaI        AflII       
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccI           22  GTAGAC               GT^[AC][GT]AC
AflIII        108  ACATGT               A^C[GA][CT]GT
AluI          171  AGCT                 AG^CT
BbvI           15  GCAGC                GCAGC(8/12)
BfaI          178  CTAG                 C^TAG
BsiYI          71  CCGAAGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           71  CCGAAGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         202  GAGAC                GTCTC(1/5)
BspLU11I      108  ACATGT               A^CATGT
BsrFI          18  GCCGGT               [GA]^CCGG[CT]
BsrI           77  ACTGG                ACTGG(1/-1)
Cac8I          82  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I         18  GCCGGT               [GA]^CCGG[CT]
EarI           58  GAAGAG               CTCTTC(1/4)
Fnu4HI         15  GCAGC                GC^[ACGT]GC
HapII          19  CCGG                 C^CGG
HpaII          19  CCGG                 C^CGG
ItaI           15  GCAGC                GC^[ACGT]GC
Ksp632I        58  GAAGAG               CTCTTC(1/4)
MaeI          178  CTAG                 C^TAG
MaeIII        165  GTTAC                ^GT[ACGT]AC
MspI           19  CCGG                 C^CGG
NspI          108  ACATGT               [GA]CATG^[CT]
PshAI          25  GACTTAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI         103  GATGC                GCATC(5/9)
TseI           15  GCAGC                G^C[AT]GC
Tsp509I       124  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BbsI           43  GAAGAC               GAAGAC(2/6)
               73  GAAGAC              
BpuAI          43  GAAGAC               GAAGAC(2/6)
               73  GAAGAC              
BstF5I        129  GGATG                GGATG(2/0)
              147  GGATG               
DdeI           27  CTTAG                C^T[ACGT]AG
               87  CTTAG               
FokI          129  GGATG                GGATG(9/13)
              147  GGATG               
NlaIII        109  CATG                 CATG^
              187  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       MnlI