##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MboI        MboII       MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI          177  GCGG                 CCGC(-3/-1)
AcsI          151  GAATTC               [GA]^AATT[CT]
AfaI          218  GTAC                 GT^AC
AflIII         70  ACACGT               A^C[GA][CT]GT
ApoI          151  GAATTC               [GA]^AATT[CT]
AspHI          28  GTGCTC               G[AT]GC[AT]^C
AvaII         131  GGACC                G^G[AT]CC
BbrPI          71  CACGTG               CAC^GTG
BmyI           28  GTGCTC               G[AGT]GC[ACT]^C
BsaAI          71  CACGTG               [CT]AC^GT[GA]
BsiHKAI        28  GTGCTC               G[AT]GC[AT]^C
BsmAI          50  GTCTC                GTCTC(1/5)
Bsp1286I       28  GTGCTC               G[AGT]GC[ACT]^C
BspMI          94  ACCTGC               ACCTGC(4/8)
BsrI          158  CCAGT                ACTGG(1/-1)
BstF5I        223  CATCC                GGATG(2/0)
Cac8I         177  GCGGGC               GC[ACGT]^[ACGT]GC
DdeI           46  CTGAG                C^T[ACGT]AG
EcoRI         151  GAATTC               G^AATTC
FauI          177  GCGGG                CCCGC(4/6)
FokI          223  CATCC                GGATG(9/13)
HinfI          48  GAGTC                G^A[ACGT]TC
HphI          155  TCACC                GGTGA(8/7)
MaeII          72  ACGT                 A^CGT
MfeI          277  CAATTG               C^AATTG
MunI          277  CAATTG               C^AATTG
PleI           48  GAGTC                GAGTC(4/5)
PmaCI          71  CACGTG               CAC^GTG
PmlI           71  CACGTG               CAC^GTG
PvuII         165  CAGCTG               CAG^CTG
RsaI          218  GTAC                 GT^AC
Tsp45I         80  GTGAC                ^GT[GC]AC
XcmI          226  CCAAGGAAAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          145  AGCT                 AG^CT
              166  AGCT                
BbvI           98  GCTGC                GCAGC(8/12)
              167  GCTGC               
Cfr13I          6  GGGCC                G^G[ACGT]CC
              131  GGACC               
Fnu4HI         98  GCTGC                GC^[ACGT]GC
              167  GCTGC               
ItaI           98  GCTGC                GC^[ACGT]GC
              167  GCTGC               
MaeIII         80  GTGAC                ^GT[ACGT]AC
              161  GTAAC               
MspA1I        165  CAGCTG               C[AC]G^C[GT]G
              175  CAGCGG              
Sau96I          6  GGGCC                G^G[ACGT]CC
              131  GGACC               
TseI           98  GCTGC                G^C[AT]GC
              167  GCTGC               
Tsp509I       152  AATT                 ^AATT
              278  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BssKI       BstNI       
EcoRII      EcoT14I     HaeIII      MnlI        MvaI        
ScrFI       StyI