Restriction map for
pFN21AE1737
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 281 GGTACC G^GTACC
AccII 607 CGCG CG^CG
AfaI 282 GTAC GT^AC
Aor51HI 36 AGCGCT AGC^GCT
Asp718I 281 GGTACC G^GTACC
AvaI 6 CTCGGG C^[CT]CG[GA]G
BalI 193 TGGCCA TGG^CCA
BamHI 264 GGATCC G^GATCC
BanII 3 GAGCTC G[GA]GC[CT]^C
BbsI 510 GAAGAC GAAGAC(2/6)
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BpuAI 510 GAAGAC GAAGAC(2/6)
BsaAI 697 TACGTG [CT]AC^GT[GA]
BsaI 529 GGTCTC GGTCTC(1/5)
BsaWI 690 ACCGGA [AT]^CCGG[AT]
BseRI 676 GAGGAG GAGGAG(10/8)
BsoBI 6 CTCGGG C^[CT]CG[GA]G
BsrDI 339 CATTGC GCAATG(2/0)
BstUI 607 CGCG CG^CG
BstXI 338 CCATTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 264 GGATCC [GA]^GATC[CT]
DpnII 265 GATC ^GATC
DsaI 344 CCATGG C^C[GA][CT]GG
EaeI 193 TGGCCA [CT]^GGCC[GA]
Eco47III 36 AGCGCT AGC^GCT
HaeII 36 AGCGCT [GA]GCGC^[CT]
KpnI 281 GGTACC GGTAC^C
MaeII 698 ACGT A^CGT
MaeIII 498 GTCAC ^GT[ACGT]AC
MboI 265 GATC ^GATC
MflI 264 GGATCC [GA]^GATC[CT]
MluNI 193 TGGCCA TGG^CCA
MscI 193 TGGCCA TGG^CCA
MslI 339 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 607 CGCG CG^CG
NcoI 344 CCATGG C^CATGG
NdeII 265 GATC ^GATC
PleI 610 GAGTC GAGTC(4/5)
PshAI 308 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 477 CTGCAG CTGCA^G
RsaI 282 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
Sau3AI 265 GATC ^GATC
SfcI 477 CTGCAG C^T[GA][CT]AG
TaqI 373 TCGA T^CGA
TfiI 565 GAATC G^A[AT]TC
Tsp45I 498 GTCAC ^GT[GC]AC
TthHB8I 373 TCGA T^CGA
XcmI 614 CCAGGAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 264 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 208 GCGG CCGC(-3/-1)
366 GCGG
AlwI 265 GATCC GGATC(4/5)
264 GGATC
AspHI 3 GAGCTC G[AT]GC[AT]^C
415 GTGCTC
BpmI 634 CTCCAG CTGGAG(16/14)
259 CTGGAG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
415 GTGCTC
BsmAI 442 GAGAC GTCTC(1/5)
530 GTCTC
Cac8I 186 GCAGGC GC[ACGT]^[ACGT]GC
425 GCAAGC
EcoT14I 176 CCTTGG C^C[AT][AT]GG
344 CCATGG
HinfI 565 GAATC G^A[ACGT]TC
610 GAGTC
StyI 176 CCTTGG C^C[AT][AT]GG
344 CCATGG
Tsp509I 245 AATT ^AATT
400 AATT
TspRI 500 CACTG CA[GC]TG[ACGT][ACGT]^
601 CAGTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AvaII BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PpuMI Sau96I ScrFI
SfaNI TseI