Restriction map for
pFN21AE0718
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HindIII MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 673 GGTACC G^GTACC
AcyI 43 GGCGCC G[GA]^CG[CT]C
AocI 50 CCTCAGG CC^T[ACGT]AGG
Asp718I 673 GGTACC G^GTACC
AspHI 655 GAGCTC G[AT]GC[AT]^C
AviII 149 TGCGCA TGC^GCA
BbeI 43 GGCGCC GGCGC^C
BciVI 1062 GGATAC GTATCC(6/5)
BsaHI 43 GGCGCC G[GA]^CG[CT]C
BseRI 33 CTCCTC GAGGAG(10/8)
BsiEI 94 CGGCCG CG[GA][CT]^CG
BsiHKAI 655 GAGCTC G[AT]GC[AT]^C
BsmAI 385 GAGAC GTCTC(1/5)
BsrBI 81 GAGCGG CCGCTC(-3/-3)
BsrDI 794 GCAATG GCAATG(2/0)
BsrFI 92 GCCGGC [GA]^CCGG[CT]
BsrI 444 CCAGT ACTGG(1/-1)
BssSI 536 CTCGTG CACGAG(-5/-1)
Bsu36I 50 CCTCAGG CC^T[ACGT]AGG
Cfr10I 92 GCCGGC [GA]^CCGG[CT]
EaeI 94 CGGCCG [CT]^GGCC[GA]
EagI 94 CGGCCG C^GGCCG
EclXI 94 CGGCCG C^GGCCG
Eco52I 94 CGGCCG C^GGCCG
Eco81I 50 CCTCAGG CC^T[ACGT]AGG
EcoNI 784 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 355 CCTTGG C^C[AT][AT]GG
FspI 149 TGCGCA TGC^GCA
HaeII 43 GGCGCC [GA]GCGC^[CT]
Hin1I 43 GGCGCC G[GA]^CG[CT]C
HpaI 753 GTTAAC GTT^AAC
KasI 43 GGCGCC G^GCGCC
KpnI 673 GGTACC GGTAC^C
KspI 117 CCGCGG CCGC^GG
NaeI 92 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NdeI 758 CATATG CA^TATG
NgoMIV 92 GCCGGC G^CCGGC
PleI 874 GAGTC GAGTC(4/5)
SacI 655 GAGCTC GAGCT^C
SacII 117 CCGCGG CCGC^GG
SapI 652 GAAGAGC GCTCTTC(1/4)
SfaNI 391 GCATC GCATC(5/9)
SfcI 174 CTACAG C^T[GA][CT]AG
SfoI 43 GGCGCC GGC^GCC
SmaI 186 CCCGGG CCC^GGG
StyI 355 CCTTGG C^C[AT][AT]GG
SwaI 1191 ATTTAAAT ATTT^AAAT
TaqI 924 TCGA T^CGA
Tsp45I 602 GTGAC ^GT[GC]AC
TthHB8I 924 TCGA T^CGA
XmaCI 186 CCCGGG C^CCGGG
XmaI 186 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 118 CGCG CG^CG
139 CGCG
AfaI 331 GTAC GT^AC
674 GTAC
AluI 197 AGCT AG^CT
656 AGCT
AlwI 3 GATCC GGATC(4/5)
712 GATCC
AseI 364 ATTAAT AT^TAAT
1054 ATTAAT
AsnI 364 ATTAAT AT^TAAT
1054 ATTAAT
Asp700I 310 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
514 GAAGGTGTTC
AvaI 57 CTCGGG C^[CT]CG[GA]G
186 CCCGGG
BanI 43 GGCGCC G^G[CT][GA]CC
673 GGTACC
BanII 157 GAGCCC G[GA]GC[CT]^C
655 GAGCTC
BglI 62 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
87 GCCGTGCCGGC
BsoBI 57 CTCGGG C^[CT]CG[GA]G
186 CCCGGG
BspHI 272 TCATGA T^CATGA
318 TCATGA
BstF5I 305 GGATG GGATG(2/0)
995 GGATG
BstUI 118 CGCG CG^CG
139 CGCG
DraI 472 TTTAAA TTT^AAA
1192 TTTAAA
DsaI 117 CCGCGG C^C[GA][CT]GG
143 CCGTGG
EarI 652 GAAGAG CTCTTC(1/4)
1102 GAAGAG
FokI 305 GGATG GGATG(9/13)
995 GGATG
Ksp632I 652 GAAGAG CTCTTC(1/4)
1102 GAAGAG
MslI 532 CACACTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
744 CATTGGAGTG
MspA1I 24 CAGCGG C[AC]G^C[GT]G
117 CCGCGG
MvnI 118 CGCG CG^CG
139 CGCG
PshBI 364 ATTAAT AT^TAAT
1054 ATTAAT
RcaI 272 TCATGA T^CATGA
318 TCATGA
RsaI 331 GTAC GT^AC
674 GTAC
SfiI 61 GGCCGGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
86 GGCCGTGCCGGCC
SspI 571 AATATT AAT^ATT
636 AATATT
XmnI 310 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
514 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI ApoI BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I TspRI