Restriction map for
pFN21AE4032
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BseRI BsgI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MaeI
MaeII MaeIII MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 493 AAATTT [GA]^AATT[CT]
AflIII 458 ACATGT A^C[GA][CT]GT
AluI 441 AGCT AG^CT
Alw44I 349 GTGCAC G^TGCAC
AlwI 63 GGATC GGATC(4/5)
AocI 361 CCTGAGG CC^T[ACGT]AGG
ApaI 278 GGGCCC GGGCC^C
ApaLI 349 GTGCAC G^TGCAC
ApoI 493 AAATTT [GA]^AATT[CT]
BciVI 499 GTATCC GTATCC(6/5)
BglII 700 AGATCT A^GATCT
BsaBI 517 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 329 CGTACG C^GTACG
BspLU11I 458 ACATGT A^CATGT
BsrI 74 CCAGT ACTGG(1/-1)
BstNI 420 CCTGG CC^[AT]GG
Bsu36I 361 CCTGAGG CC^T[ACGT]AGG
DraII 277 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 641 GACTGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 361 CCTGAGG CC^T[ACGT]AGG
EcoO109I 277 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 420 CCTGG ^CC[AT]GG
EcoT22I 654 ATGCAT ATGCA^T
HgaI 271 GCGTC GACGC(5/10)
HinfI 343 GACTC G^A[ACGT]TC
MamI 517 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 610 GAAGA GAAGA(8/7)
MvaI 420 CCTGG CC^[AT]GG
NlaIII 459 CATG CATG^
NotI 199 GCGGCCGC GC^GGCCGC
NsiI 654 ATGCAT ATGCA^T
NspI 458 ACATGT [GA]CATG^[CT]
PleI 343 GACTC GAGTC(4/5)
SfcI 632 CTATAG C^T[GA][CT]AG
SfiI 201 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 349 GTGCAC G[AT]GC[AT]^C
712 GAGCAC
AvaI 54 CTCGGG C^[CT]CG[GA]G
122 CTCGGG
AvaII 98 GGACC G^G[AT]CC
384 GGACC
BsiHKAI 349 GTGCAC G[AT]GC[AT]^C
712 GAGCAC
BsoBI 54 CTCGGG C^[CT]CG[GA]G
122 CTCGGG
BsrBI 240 CCGCTC CCGCTC(-3/-3)
15 GAGCGG
BsrFI 386 ACCGGC [GA]^CCGG[CT]
390 GCCGGT
BssHII 45 GCGCGC G^CGCGC
164 GCGCGC
BstF5I 306 CATCC GGATG(2/0)
567 GGATG
BstYI 63 GGATCT [GA]^GATC[CT]
700 AGATCT
Cfr10I 386 ACCGGC [GA]^CCGG[CT]
390 GCCGGT
FokI 306 CATCC GGATG(9/13)
567 GGATG
HphI 423 GGTGA GGTGA(8/7)
444 TCACC
MflI 63 GGATCT [GA]^GATC[CT]
700 AGATCT
MseI 473 TTAA T^TAA
538 TTAA
Tru9I 473 TTAA T^TAA
538 TTAA
Tsp509I 494 AATT ^AATT
614 AATT
XhoII 63 GGATCT [GA]^GATC[CT]
700 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AfaI BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI Bsp1286I BssKI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MboI MnlI MspA1I
MspI MvnI MwoI NarI NciI
NdeII NlaIV RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI TseI