Restriction map for
pFN21AE3414
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BbeI
BbsI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MslI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 651 AAATTT [GA]^AATT[CT]
AflII 81 CTTAAG C^TTAAG
ApoI 651 AAATTT [GA]^AATT[CT]
Asp700I 16 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 162 GGATCC G^GATCC
BanII 317 GGGCTC G[GA]GC[CT]^C
BbrPI 635 CACGTG CAC^GTG
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
BcnI 778 CCCGG CC^[GC]GG
BfrI 81 CTTAAG C^TTAAG
BmyI 317 GGGCTC G[AGT]GC[ACT]^C
BpmI 752 CTGGAG CTGGAG(16/14)
BsaAI 635 CACGTG [CT]AC^GT[GA]
BsmFI 984 GGGAC GGGAC(10/14)
Bsp1286I 317 GGGCTC G[AGT]GC[ACT]^C
Cac8I 169 GCAAGC GC[ACGT]^[ACGT]GC
DraIII 635 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
HapII 779 CCGG C^CGG
HpaII 779 CCGG C^CGG
HphI 378 TCACC GGTGA(8/7)
MfeI 254 CAATTG C^AATTG
MspI 779 CCGG C^CGG
MunI 254 CAATTG C^AATTG
NciI 778 CCCGG CC^[GC]GG
NdeI 989 CATATG CA^TATG
PflMI 988 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 635 CACGTG CAC^GTG
PmlI 635 CACGTG CAC^GTG
SfaNI 226 GATGC GCATC(5/9)
SfcI 287 CTGTAG C^T[GA][CT]AG
SmlI 81 CTTAAG C^T[CT][GA]AG
Van91I 988 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 565 TCTAGA T^CTAGA
XcmI 522 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 16 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 350 GCGG CCGC(-3/-1)
644 GCGG
BbvI 229 GCTGC GCAGC(8/12)
238 GCTGC
BfaI 566 CTAG C^TAG
596 CTAG
BstF5I 209 CATCC GGATG(2/0)
723 GGATG
BstYI 162 GGATCC [GA]^GATC[CT]
688 GGATCT
DraII 747 AGGACCT [GA]G^G[ACGT]CC[CT]
816 AGGACCT
DsaI 212 CCATGG C^C[GA][CT]GG
233 CCATGG
EcoO109I 747 AGGACCT [GA]G^G[ACGT]CC[CT]
816 AGGACCT
EcoT14I 212 CCATGG C^C[AT][AT]GG
233 CCATGG
Fnu4HI 229 GCTGC GC^[ACGT]GC
238 GCTGC
FokI 209 CATCC GGATG(9/13)
723 GGATG
HindIII 84 AAGCTT A^AGCTT
136 AAGCTT
HinfI 301 GAATC G^A[ACGT]TC
631 GATTC
ItaI 229 GCTGC GC^[ACGT]GC
238 GCTGC
MaeI 566 CTAG C^TAG
596 CTAG
MflI 162 GGATCC [GA]^GATC[CT]
688 GGATCT
MseI 82 TTAA T^TAA
356 TTAA
NcoI 212 CCATGG C^CATGG
233 CCATGG
PpuMI 747 AGGACCT [GA]G^G[AT]CC[CT]
816 AGGACCT
StyI 212 CCATGG C^C[AT][AT]GG
233 CCATGG
TfiI 301 GAATC G^A[AT]TC
631 GATTC
Tru9I 82 TTAA T^TAA
356 TTAA
TseI 229 GCTGC G^C[AT]GC
238 GCTGC
TspRI 705 CAGTG CA[GC]TG[ACGT][ACGT]^
1020 CAGTG
XhoII 162 GGATCC [GA]^GATC[CT]
688 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI AvaII BsaJI BseRI
BsiYI BslI BssKI BstNI Cfr13I
DdeI DpnII EarI EcoRII Ksp632I
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tsp509I