Restriction map for
pFN21AE1805
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BfaI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 39 CGCG CG^CG
AflII 955 CTTAAG C^TTAAG
AscI 37 GGCGCGCC GG^CGCGCC
Asp700I 487 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 441 GGTCC G^G[AT]CC
BbrPI 127 CACGTG CAC^GTG
BbsI 579 GAAGAC GAAGAC(2/6)
BclI 368 TGATCA T^GATCA
BfrI 955 CTTAAG C^TTAAG
BpmI 853 CTCCAG CTGGAG(16/14)
BpuAI 579 GAAGAC GAAGAC(2/6)
BsaAI 127 CACGTG [CT]AC^GT[GA]
BseRI 465 CTCCTC GAGGAG(10/8)
BsmAI 150 GTCTC GTCTC(1/5)
BsmFI 168 GTCCC GGGAC(10/14)
BspMI 64 ACCTGC ACCTGC(4/8)
BsrBI 768 CCGCTC CCGCTC(-3/-3)
BsrDI 636 CATTGC GCAATG(2/0)
BsrFI 74 ACCGGC [GA]^CCGG[CT]
BssHII 38 GCGCGC G^CGCGC
BstAPI 414 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 39 CGCG CG^CG
BstXI 308 CCAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 74 ACCGGC [GA]^CCGG[CT]
DraI 186 TTTAAA TTT^AAA
DraII 440 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 449 CCACGG C^C[GA][CT]GG
EcoO109I 440 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 324 GAATTC G^AATTC
EcoT14I 799 CCTTGG C^C[AT][AT]GG
FbaI 368 TGATCA T^GATCA
HgaI 582 GACGC GACGC(5/10)
HincII 682 GTCAAC GT[CT]^[GA]AC
HindII 682 GTCAAC GT[CT]^[GA]AC
HphI 821 TCACC GGTGA(8/7)
MslI 63 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 39 CGCG CG^CG
NspI 68 GCATGT [GA]CATG^[CT]
PmaCI 127 CACGTG CAC^GTG
PmlI 127 CACGTG CAC^GTG
PpuMI 440 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 544 CAGCTG CAG^CTG
SapI 792 GAAGAGC GCTCTTC(1/4)
SexAI 11 ACCAGGT A^CC[AT]GGT
StyI 799 CCTTGG C^C[AT][AT]GG
TaqI 620 TCGA T^CGA
Tsp45I 877 GTGAC ^GT[GC]AC
TthHB8I 620 TCGA T^CGA
XcmI 305 CCACCAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 487 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 324 GAATTC [GA]^AATT[CT]
592 AAATTC
Alw44I 88 GTGCAC G^TGCAC
454 GTGCAC
ApaLI 88 GTGCAC G^TGCAC
454 GTGCAC
ApoI 324 GAATTC [GA]^AATT[CT]
592 AAATTC
BalI 123 TGGCCA TGG^CCA
663 TGGCCA
BanII 289 GAGCCC G[GA]GC[CT]^C
795 GAGCCC
BcnI 115 CCGGG CC^[GC]GG
381 CCCGG
BstNI 12 CCAGG CC^[AT]GG
924 CCTGG
DpnII 369 GATC ^GATC
861 GATC
EaeI 123 TGGCCA [CT]^GGCC[GA]
663 TGGCCA
EarI 136 GAAGAG CTCTTC(1/4)
792 GAAGAG
EcoRII 12 CCAGG ^CC[AT]GG
924 CCTGG
FauI 170 CCCGC CCCGC(4/6)
530 CCCGC
Ksp632I 136 GAAGAG CTCTTC(1/4)
792 GAAGAG
MaeIII 265 GTAAC ^GT[ACGT]AC
877 GTGAC
MboI 369 GATC ^GATC
861 GATC
MluNI 123 TGGCCA TGG^CCA
663 TGGCCA
MscI 123 TGGCCA TGG^CCA
663 TGGCCA
MspA1I 544 CAGCTG C[AC]G^C[GT]G
758 CCGCTG
MvaI 12 CCAGG CC^[AT]GG
924 CCTGG
NciI 115 CCGGG CC^[GC]GG
381 CCCGG
NdeII 369 GATC ^GATC
861 GATC
NlaIII 69 CATG CATG^
774 CATG
PleI 139 GAGTC GAGTC(4/5)
166 GAGTC
PstI 212 CTGCAG CTGCA^G
870 CTGCAG
Sau3AI 369 GATC ^GATC
861 GATC
SfaNI 250 GATGC GCATC(5/9)
599 GCATC
SfcI 212 CTGCAG C^T[GA][CT]AG
870 CTGCAG
TfiI 237 GATTC G^A[AT]TC
551 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I Cac8I
CfoI Cfr13I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MboII MnlI
MseI MspI MwoI NlaIV RsaI
Sau96I ScrFI SmlI Tru9I TseI
Tsp509I TspRI