Restriction map for
pFN21AE5283
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeI
MamI MluI MluNI MscI MslI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 19 CGCG CG^CG
AccIII 174 TCCGGA T^CCGGA
AcsI 149 AAATTC [GA]^AATT[CT]
AhdI 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 261 GGATC GGATC(4/5)
AlwNI 622 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 23 GGGCCC GGGCC^C
ApoI 149 AAATTC [GA]^AATT[CT]
AspEI 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 23 GGGCCC G[GA]GC[CT]^C
BbsI 736 GTCTTC GAAGAC(2/6)
BmyI 23 GGGCCC G[AGT]GC[ACT]^C
BpuAI 736 GTCTTC GAAGAC(2/6)
BsaI 167 GAGACC GGTCTC(1/5)
BsaWI 174 TCCGGA [AT]^CCGG[AT]
BseAI 174 TCCGGA T^CCGGA
BsgI 636 CTGCAC GTGCAG(16/14)
Bsp1286I 23 GGGCCC G[AGT]GC[ACT]^C
BspEI 174 TCCGGA T^CCGGA
BspMI 358 ACCTGC ACCTGC(4/8)
BstUI 19 CGCG CG^CG
BstYI 261 GGATCT [GA]^GATC[CT]
DraI 502 TTTAAA TTT^AAA
Eam1105I 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 250 GAAGAG CTCTTC(1/4)
FauI 20 GCGGG CCCGC(4/6)
HaeII 77 AGCGCC [GA]GCGC^[CT]
HinfI 716 GAGTC G^A[ACGT]TC
Ksp632I 250 GAAGAG CTCTTC(1/4)
MaeII 296 ACGT A^CGT
MaeIII 86 GTGAC ^GT[ACGT]AC
MfeI 584 CAATTG C^AATTG
MflI 261 GGATCT [GA]^GATC[CT]
MroI 174 TCCGGA T^CCGGA
MspA1I 625 CAGCTG C[AC]G^C[GT]G
MunI 584 CAATTG C^AATTG
MvnI 19 CGCG CG^CG
PleI 716 GAGTC GAGTC(4/5)
PpuMI 355 GGGACCT [GA]G^G[AT]CC[CT]
PstI 8 CTGCAG CTGCA^G
PvuII 625 CAGCTG CAG^CTG
ScaI 398 AGTACT AGT^ACT
Tsp45I 86 GTGAC ^GT[GC]AC
XhoII 261 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 399 GTAC GT^AC
577 GTAC
AluI 431 AGCT AG^CT
626 AGCT
AvaII 356 GGACC G^G[AT]CC
435 GGACC
BcnI 646 CCGGG CC^[GC]GG
705 CCGGG
BsmAI 133 GAGAC GTCTC(1/5)
167 GAGAC
BsmFI 355 GGGAC GGGAC(10/14)
660 GTCCC
Cac8I 80 GCCTGC GC[ACGT]^[ACGT]GC
681 GCTGGC
DpnII 230 GATC ^GATC
262 GATC
MboI 230 GATC ^GATC
262 GATC
MseI 257 TTAA T^TAA
503 TTAA
NciI 646 CCGGG CC^[GC]GG
705 CCGGG
NdeII 230 GATC ^GATC
262 GATC
RsaI 399 GTAC GT^AC
577 GTAC
Sau3AI 230 GATC ^GATC
262 GATC
SfaNI 376 GATGC GCATC(5/9)
508 GATGC
Tru9I 257 TTAA T^TAA
503 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI SfcI TseI Tsp509I
TspRI