Restriction map for
pFN21AE6630
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsgI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MluI MluNI MscI
MslI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 619 TCCGGA T^CCGGA
AciI 82 CCGC CCGC(-3/-1)
AcsI 220 AAATTC [GA]^AATT[CT]
AfaI 735 GTAC GT^AC
Alw44I 91 GTGCAC G^TGCAC
AlwNI 517 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 698 AGCGCT AGC^GCT
ApaLI 91 GTGCAC G^TGCAC
ApoI 220 AAATTC [GA]^AATT[CT]
AspHI 91 GTGCAC G[AT]GC[AT]^C
BanI 558 GGTGCC G^G[CT][GA]CC
BcnI 87 CCCGG CC^[GC]GG
BmyI 91 GTGCAC G[AGT]GC[ACT]^C
BsaAI 736 TACGTG [CT]AC^GT[GA]
BsaBI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 16 GAGACC GGTCTC(1/5)
BsaJI 554 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 619 TCCGGA [AT]^CCGG[AT]
BseAI 619 TCCGGA T^CCGGA
BseRI 707 GAGGAG GAGGAG(10/8)
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
BsmBI 623 GAGACG CGTCTC(1/5)
Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C
BspEI 619 TCCGGA T^CCGGA
BsrBI 82 CCGCTC CCGCTC(-3/-3)
BsrI 684 ACTGG ACTGG(1/-1)
BstXI 62 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 422 AGATCC [GA]^GATC[CT]
CfoI 699 GCGC GCG^C
DraI 184 TTTAAA TTT^AAA
Eco47III 698 AGCGCT AGC^GCT
EcoT14I 554 CCTTGG C^C[AT][AT]GG
HaeII 698 AGCGCT [GA]GCGC^[CT]
HhaI 699 GCGC GCG^C
HinP1I 699 GCGC G^CGC
HphI 235 GGTGA GGTGA(8/7)
MaeII 737 ACGT A^CGT
MamI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 245 CAATTG C^AATTG
MflI 422 AGATCC [GA]^GATC[CT]
MroI 619 TCCGGA T^CCGGA
MunI 245 CAATTG C^AATTG
NciI 87 CCCGG CC^[GC]GG
NspI 398 ACATGC [GA]CATG^[CT]
PflMI 516 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 754 GAGTC GAGTC(4/5)
PpuMI 509 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 173 CTGCAG CTGCA^G
RsaI 735 GTAC GT^AC
StyI 554 CCTTGG C^C[AT][AT]GG
TspRI 683 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 516 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 422 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwI 423 GATCC GGATC(4/5)
672 GATCC
BbvI 66 GCTGC GCAGC(8/12)
172 GCTGC
BsiYI 169 CCAGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
516 CCAGCATCTGG
BslI 169 CCAGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
516 CCAGCATCTGG
BsmFI 665 GGGAC GGGAC(10/14)
742 GTCCC
BstBI 215 TTCGAA TT^CGAA
264 TTCGAA
BstNI 284 CCTGG CC^[AT]GG
675 CCAGG
DpnII 423 GATC ^GATC
672 GATC
DraII 162 AGGCCCT [GA]G^G[ACGT]CC[CT]
509 AGGTCCT
EarI 54 GAAGAG CTCTTC(1/4)
648 GAAGAG
EcoO109I 162 AGGCCCT [GA]G^G[ACGT]CC[CT]
509 AGGTCCT
EcoRII 284 CCTGG ^CC[AT]GG
675 CCAGG
HapII 88 CCGG C^CGG
620 CCGG
HpaII 88 CCGG C^CGG
620 CCGG
Ksp632I 54 GAAGAG CTCTTC(1/4)
648 GAAGAG
MaeIII 236 GTGAC ^GT[ACGT]AC
499 GTCAC
MboI 423 GATC ^GATC
672 GATC
MboII 54 GAAGA GAAGA(8/7)
648 GAAGA
MspA1I 170 CAGCTG C[AC]G^C[GT]G
533 CAGCTG
MspI 88 CCGG C^CGG
620 CCGG
MvaI 284 CCTGG CC^[AT]GG
675 CCAGG
MwoI 84 GCTCCCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
172 GCTGCAGGGGC
NdeII 423 GATC ^GATC
672 GATC
NlaIV 558 GGTGCC GG[ACGT]^[ACGT]CC
741 GGTCCC
NspV 215 TTCGAA TT^CGAA
264 TTCGAA
PvuII 170 CAGCTG CAG^CTG
533 CAGCTG
Sau3AI 423 GATC ^GATC
672 GATC
SfaNI 48 GATGC GCATC(5/9)
519 GCATC
SfcI 173 CTGCAG C^T[GA][CT]AG
636 CTACAG
SfuI 215 TTCGAA TT^CGAA
264 TTCGAA
SmlI 253 CTTGAG C^T[CT][GA]AG
505 CTCAAG
StuI 551 AGGCCT AGG^CCT
689 AGGCCT
TfiI 262 GATTC G^A[AT]TC
389 GAATC
TseI 66 GCTGC G^C[AT]GC
172 GCTGC
Tsp45I 236 GTGAC ^GT[GC]AC
499 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AvaII BpmI BsmAI BssKI
Cac8I Cfr13I DdeI Fnu4HI HaeIII
HinfI ItaI MnlI MseI NlaIII
Sau96I ScrFI TaqI Tru9I Tsp509I
TthHB8I