Restriction map for
pFN21AE6615
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 797 GGTACC G^GTACC
AcsI 274 GAATTT [GA]^AATT[CT]
AhdI 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 53 GGATC GGATC(4/5)
ApoI 274 GAATTT [GA]^AATT[CT]
Asp718I 797 GGTACC G^GTACC
AspEI 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 745 GTGCTC G[AT]GC[AT]^C
AvaI 378 CCCGAG C^[CT]CG[GA]G
BbsI 136 GAAGAC GAAGAC(2/6)
BcgI 438 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 555 GGATAC GTATCC(6/5)
BpuAI 136 GAAGAC GAAGAC(2/6)
BsaI 266 GGTCTC GGTCTC(1/5)
BsgI 160 CTGCAC GTGCAG(16/14)
BsiHKAI 745 GTGCTC G[AT]GC[AT]^C
BsmBI 297 GAGACG CGTCTC(1/5)
BsmI 687 GAATGC GAATGC(1/-1)
BsoBI 378 CCCGAG C^[CT]CG[GA]G
BsrI 536 ACTGG ACTGG(1/-1)
BstBI 446 TTCGAA TT^CGAA
BstYI 53 GGATCT [GA]^GATC[CT]
CfoI 834 GCGC GCG^C
DraI 58 TTTAAA TTT^AAA
DraII 422 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 466 GAAGAG CTCTTC(1/4)
EcoO109I 422 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 760 CCCGC CCCGC(4/6)
HhaI 834 GCGC GCG^C
HinP1I 834 GCGC G^CGC
KpnI 797 GGTACC GGTAC^C
Ksp632I 466 GAAGAG CTCTTC(1/4)
MaeII 431 ACGT A^CGT
MflI 53 GGATCT [GA]^GATC[CT]
NheI 672 GCTAGC G^CTAGC
NspV 446 TTCGAA TT^CGAA
PflMI 849 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 12 GACTC GAGTC(4/5)
PpuMI 422 AGGACCC [GA]G^G[AT]CC[CT]
PstI 682 CTGCAG CTGCA^G
ScaI 548 AGTACT AGT^ACT
SfcI 682 CTGCAG C^T[GA][CT]AG
SfuI 446 TTCGAA TT^CGAA
Tsp509I 275 AATT ^AATT
Van91I 849 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 53 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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BanI 797 GGTACC G^G[CT][GA]CC
819 GGTGCC
BanII 386 GGGCTC G[GA]GC[CT]^C
397 GAGCCC
BfaI 101 CTAG C^TAG
673 CTAG
BsmAI 297 GAGAC GTCTC(1/5)
267 GTCTC
BsmFI 495 GGGAC GGGAC(10/14)
331 GTCCC
BspMI 158 ACCTGC ACCTGC(4/8)
691 GCAGGT
Cac8I 118 GCTCGC GC[ACGT]^[ACGT]GC
672 GCTAGC
DsaI 791 CCGTGG C^C[GA][CT]GG
815 CCACGG
HphI 699 GGTGA GGTGA(8/7)
858 GGTGA
MaeI 101 CTAG C^TAG
673 CTAG
SmlI 517 CTCAAG C^T[CT][GA]AG
829 CTCAAG
TaqI 447 TCGA T^CGA
735 TCGA
TthHB8I 447 TCGA T^CGA
735 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I TspRI