Restriction map for
pFN21AE6494
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AccII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BciVI BcnI BfrI BglI
BlnI BpuAI BsaAI BseAI BsgI
BsiEI BsiWI BsoBI BspDI BspEI
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV FauI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MluI MroI
MvnI NarI NciI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1363 GACGTC GACGT^C
Acc65I 3 GGTACC G^GTACC
AccI 1012 GTAGAC GT^[AC][GT]AC
AcyI 1363 GACGTC G[GA]^CG[CT]C
AflIII 593 ACATGT A^C[GA][CT]GT
AlwNI 1352 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 24 GGGCCC GGGCC^C
Asp718I 3 GGTACC G^GTACC
BalI 876 TGGCCA TGG^CCA
BanI 3 GGTACC G^G[CT][GA]CC
BbvCI 1298 CCTCAGC CCTCAGC(-5/-2)
BclI 755 TGATCA T^GATCA
BglII 1466 AGATCT A^GATCT
BlpI 1458 GCTCAGC GC^T[ACGT]AGC
BpmI 1841 CTCCAG CTGGAG(16/14)
Bpu1102I 1458 GCTCAGC GC^T[ACGT]AGC
BsaHI 1363 GACGTC G[GA]^CG[CT]C
BsaWI 286 ACCGGA [AT]^CCGG[AT]
BsmI 533 GCATTC GAATGC(1/-1)
Bsp1407I 864 TGTACA T^GTACA
BspHI 1607 TCATGA T^CATGA
BspLU11I 593 ACATGT A^CATGT
BsrFI 80 GCCGGC [GA]^CCGG[CT]
BsrGI 864 TGTACA T^GTACA
BstAPI 466 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1074 GGTGACC G^GT[ACGT]ACC
BstPI 1074 GGTGACC G^GT[ACGT]ACC
BstXI 1441 CCAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1458 GCTCAGC GC^T[ACGT]AGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
EaeI 876 TGGCCA [CT]^GGCC[GA]
EcoO65I 1074 GGTGACC G^GT[ACGT]ACC
EcoT22I 899 ATGCAT ATGCA^T
FbaI 755 TGATCA T^GATCA
HgaI 407 GACGC GACGC(5/10)
Hin1I 1363 GACGTC G[GA]^CG[CT]C
HincII 1191 GTCAAC GT[CT]^[GA]AC
HindII 1191 GTCAAC GT[CT]^[GA]AC
HindIII 360 AAGCTT A^AGCTT
KpnI 3 GGTACC GGTAC^C
MaeII 1364 ACGT A^CGT
MluNI 876 TGGCCA TGG^CCA
MscI 876 TGGCCA TGG^CCA
MspA1I 72 CAGCGG C[AC]G^C[GT]G
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NsiI 899 ATGCAT ATGCA^T
PflMI 1149 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 293 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1125 CTGCAG CTGCA^G
RcaI 1607 TCATGA T^CATGA
SacI 249 GAGCTC GAGCT^C
SapI 190 GCTCTTC GCTCTTC(1/4)
SfcI 1125 CTGCAG C^T[GA][CT]AG
SpeI 852 ACTAGT A^CTAGT
SspBI 864 TGTACA T^GTACA
StuI 492 AGGCCT AGG^CCT
TfiI 609 GATTC G^A[AT]TC
Van91I 1149 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsaBI 522 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
775 GATGAATATC
BsmBI 61 GAGACG CGTCTC(1/5)
1291 GAGACG
BstYI 798 GGATCT [GA]^GATC[CT]
1466 AGATCT
HapII 81 CCGG C^CGG
287 CCGG
HpaII 81 CCGG C^CGG
287 CCGG
MamI 522 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
775 GATGAATATC
MfeI 632 CAATTG C^AATTG
1427 CAATTG
MflI 798 GGATCT [GA]^GATC[CT]
1466 AGATCT
MslI 1101 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1583 CATGGGTGTG
MspI 81 CCGG C^CGG
287 CCGG
MunI 632 CAATTG C^AATTG
1427 CAATTG
XhoII 798 GGATCT [GA]^GATC[CT]
1466 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BmyI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I TspRI