Restriction map for
pFN21AE0765
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI Fnu4HI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeI
MaeII MaeIII MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI TaqI TseI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 360 CCGC CCGC(-3/-1)
AflIII 522 ACATGT A^C[GA][CT]GT
AvaII 819 GGACC G^G[AT]CC
BalI 194 TGGCCA TGG^CCA
BanI 791 GGTGCC G^G[CT][GA]CC
BanII 318 GAGCTC G[GA]GC[CT]^C
BglII 247 AGATCT A^GATCT
BpmI 481 CTGGAG CTGGAG(16/14)
BseRI 536 GAGGAG GAGGAG(10/8)
BsmFI 448 GTCCC GGGAC(10/14)
BspLU11I 522 ACATGT A^CATGT
BsrI 50 ACTGG ACTGG(1/-1)
BstF5I 983 CATCC GGATG(2/0)
BstXI 169 CCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 500 GCGC GCG^C
Cfr13I 819 GGACC G^G[ACGT]CC
DsaI 191 CCATGG C^C[GA][CT]GG
EaeI 194 TGGCCA [CT]^GGCC[GA]
EarI 505 GAAGAG CTCTTC(1/4)
EcoT22I 911 ATGCAT ATGCA^T
FokI 983 CATCC GGATG(9/13)
HaeII 499 GGCGCT [GA]GCGC^[CT]
HaeIII 195 GGCC GG^CC
HhaI 500 GCGC GCG^C
HinP1I 500 GCGC G^CGC
HinfI 979 GATTC G^A[ACGT]TC
HphI 243 GGTGA GGTGA(8/7)
Ksp632I 505 GAAGAG CTCTTC(1/4)
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MslI 983 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 454 GCTGTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 191 CCATGG C^CATGG
NsiI 911 ATGCAT ATGCA^T
NspI 522 ACATGT [GA]CATG^[CT]
PstI 531 CTGCAG CTGCA^G
SacI 318 GAGCTC GAGCT^C
Sau96I 819 GGACC G^G[ACGT]CC
SfcI 531 CTGCAG C^T[GA][CT]AG
SspI 28 AATATT AAT^ATT
TfiI 979 GATTC G^A[AT]TC
XcmI 191 CCATGGCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 379 GATCC GGATC(4/5)
607 GGATC
AseI 25 ATTAAT AT^TAAT
666 ATTAAT
AsnI 25 ATTAAT AT^TAAT
666 ATTAAT
BsaBI 402 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
676 GATGCCAATC
BsaJI 191 CCATGG C^C[ACGT][ACGT]GG
925 CCTTGG
Bsp1407I 77 TGTACA T^GTACA
808 TGTACA
BsrGI 77 TGTACA T^GTACA
808 TGTACA
BssKI 604 CCTGG ^CC[ACGT]GG
986 CCAGG
BstNI 604 CCTGG CC^[AT]GG
986 CCAGG
BstYI 247 AGATCT [GA]^GATC[CT]
607 GGATCT
EcoRII 604 CCTGG ^CC[AT]GG
986 CCAGG
EcoT14I 191 CCATGG C^C[AT][AT]GG
925 CCTTGG
MamI 402 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
676 GATGCCAATC
MflI 247 AGATCT [GA]^GATC[CT]
607 GGATCT
MvaI 604 CCTGG CC^[AT]GG
986 CCAGG
NlaIV 791 GGTGCC GG[ACGT]^[ACGT]CC
819 GGACCC
PshBI 25 ATTAAT AT^TAAT
666 ATTAAT
ScrFI 604 CCTGG CC^[ACGT]GG
986 CCAGG
SspBI 77 TGTACA T^GTACA
808 TGTACA
StyI 191 CCATGG C^C[AT][AT]GG
925 CCTTGG
XhoII 247 AGATCT [GA]^GATC[CT]
607 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BbsI BlpI BmyI Bpu1102I BpuAI
BsiHKAI Bsp1286I CelII DdeI DpnII
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI SfaNI Tru9I
Tsp509I TspRI