Restriction map for
pFN21AE5455
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BbvI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MluI MroI MspA1I
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 202 GTCGAC GT^[AC][GT]AC
AccII 429 CGCG CG^CG
AcsI 515 AAATTT [GA]^AATT[CT]
AlwI 678 GATCC GGATC(4/5)
ApoI 515 AAATTT [GA]^AATT[CT]
Asp700I 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 228 GGTGCC G^G[CT][GA]CC
BbsI 103 GTCTTC GAAGAC(2/6)
BcgI 419 GCATCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 233 CCCGG CC^[GC]GG
BpuAI 103 GTCTTC GAAGAC(2/6)
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsiEI 197 CGGTCG CG[GA][CT]^CG
BspHI 401 TCATGA T^CATGA
BspMI 715 ACCTGC ACCTGC(4/8)
BstUI 429 CGCG CG^CG
BstYI 677 AGATCC [GA]^GATC[CT]
EcoNI 497 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 280 GCGGG CCCGC(4/6)
Fnu4HI 284 GCCGC GC^[ACGT]GC
HincII 202 GTCGAC GT[CT]^[GA]AC
HindII 202 GTCGAC GT[CT]^[GA]AC
ItaI 284 GCCGC GC^[ACGT]GC
MfeI 635 CAATTG C^AATTG
MflI 677 AGATCC [GA]^GATC[CT]
MseI 839 TTAA T^TAA
MunI 635 CAATTG C^AATTG
MvnI 429 CGCG CG^CG
MwoI 231 GCCCCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 233 CCCGG CC^[GC]GG
PpuMI 621 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 623 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 401 TCATGA T^CATGA
SalI 202 GTCGAC G^TCGAC
SapI 84 GCTCTTC GCTCTTC(1/4)
TaqI 203 TCGA T^CGA
Tru9I 839 TTAA T^TAA
TthHB8I 203 TCGA T^CGA
XhoII 677 AGATCC [GA]^GATC[CT]
XmnI 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 174 GTAC GT^AC
363 GTAC
BalI 368 TGGCCA TGG^CCA
395 TGGCCA
BanII 82 GGGCTC G[GA]GC[CT]^C
136 GGGCTC
BseRI 73 CTCCTC GAGGAG(10/8)
334 CTCCTC
BsgI 160 CTGCAC GTGCAG(16/14)
563 GTGCAG
BsmAI 5 GAGAC GTCTC(1/5)
332 GTCTC
BsmFI 124 GGGAC GGGAC(10/14)
621 GGGAC
DdeI 871 CTTAG C^T[ACGT]AG
879 CTCAG
DpnII 358 GATC ^GATC
678 GATC
DraII 505 AGGCCCC [GA]G^G[ACGT]CC[CT]
621 GGGACCC
EarI 85 CTCTTC CTCTTC(1/4)
900 GAAGAG
EcoO109I 505 AGGCCCC [GA]G^G[ACGT]CC[CT]
621 GGGACCC
EcoT14I 58 CCAAGG C^C[AT][AT]GG
797 CCAAGG
HapII 234 CCGG C^CGG
599 CCGG
HpaII 234 CCGG C^CGG
599 CCGG
Ksp632I 85 CTCTTC CTCTTC(1/4)
900 GAAGAG
MaeII 101 ACGT A^CGT
365 ACGT
MboI 358 GATC ^GATC
678 GATC
MluNI 368 TGGCCA TGG^CCA
395 TGGCCA
MscI 368 TGGCCA TGG^CCA
395 TGGCCA
MspI 234 CCGG C^CGG
599 CCGG
NdeII 358 GATC ^GATC
678 GATC
RsaI 174 GTAC GT^AC
363 GTAC
Sau3AI 358 GATC ^GATC
678 GATC
SfcI 501 CTGTAG C^T[GA][CT]AG
864 CTGTAG
StyI 58 CCAAGG C^C[AT][AT]GG
797 CCAAGG
TfiI 63 GATTC G^A[AT]TC
468 GATTC
Tsp45I 322 GTCAC ^GT[GC]AC
433 GTGAC
TspRI 146 CACTG CA[GC]TG[ACGT][ACGT]^
447 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AvaII BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I EaeI
EcoRII FokI HaeIII HinfI HphI
MaeIII MboII MnlI MslI MvaI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI Tsp509I