##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AclI          346  AACGTT               AA^CGTT
BbsI          139  GAAGAC               GAAGAC(2/6)
BbvI          334  GCAGC                GCAGC(8/12)
BcgI          365  GCAAGAAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         139  GAAGAC               GAAGAC(2/6)
BseRI          97  GAGGAG               GAGGAG(10/8)
BsiYI          37  CCGTTGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           37  CCGTTGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         423  GAGACG               CGTCTC(1/5)
BsrI           68  CCAGT                ACTGG(1/-1)
BstAPI        261  GCATGACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI            7  GCGC                 GCG^C
EaeI          356  CGGCCA               [CT]^GGCC[GA]
EarI          109  GAAGAG               CTCTTC(1/4)
Fnu4HI        334  GCAGC                GC^[ACGT]GC
HaeIII        357  GGCC                 GG^CC
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HinfI         309  GAGTC                G^A[ACGT]TC
ItaI          334  GCAGC                GC^[ACGT]GC
Ksp632I       109  GAAGAG               CTCTTC(1/4)
MaeII         347  ACGT                 A^CGT
MslI          405  CATAAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI          309  GAGTC                GAGTC(4/5)
Psp1406I      346  AACGTT               AA^CGTT
PstI          332  CTGCAG               CTGCA^G
SfcI          332  CTGCAG               C^T[GA][CT]AG
SphI           86  GCATGC               GCATG^C
TseI          334  GCAGC                G^C[AT]GC
Tsp45I        311  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

DdeI           95  CTGAG                C^T[ACGT]AG
              307  CTGAG               
MaeIII        311  GTCAC                ^GT[ACGT]AC
              386  GTTAC               
MseI          115  TTAA                 T^TAA
              235  TTAA                
MwoI          261  GCATGACATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              328  GCAACTGCAGC         
NspI           86  GCATGC               [GA]CATG^[CT]
              266  ACATGC              
SfaNI          58  GATGC                GCATC(5/9)
              186  GCATC               
Tru9I         115  TTAA                 T^TAA
              235  TTAA                
Tsp509I       147  AATT                 ^AATT
              233  AATT                
TspRI          69  CAGTG                CA[GC]TG[ACGT][ACGT]^
              279  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BfaI        BsmAI       Cac8I       MaeI        
MboII       MnlI        NlaIII