Restriction map for
pFN21AE3104
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaII ItaI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TseI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 55 GAATTC [GA]^AATT[CT]
AluI 497 AGCT AG^CT
AlwI 405 GATCC GGATC(4/5)
ApoI 55 GAATTC [GA]^AATT[CT]
BalI 15 TGGCCA TGG^CCA
BbsI 97 GAAGAC GAAGAC(2/6)
BfaI 655 CTAG C^TAG
BmyI 246 GTGCCC G[AGT]GC[ACT]^C
BpuAI 97 GAAGAC GAAGAC(2/6)
BsaBI 405 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 289 CCTTGG C^C[ACGT][ACGT]GG
BsiYI 735 CCAACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 735 CCAACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 378 GAATGC GAATGC(1/-1)
Bsp1286I 246 GTGCCC G[AGT]GC[ACT]^C
BsrDI 621 CATTGC GCAATG(2/0)
BssKI 722 CCAGG ^CC[ACGT]GG
BstNI 722 CCAGG CC^[AT]GG
BstYI 404 AGATCC [GA]^GATC[CT]
DraIII 42 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 55 GAATTC G^AATTC
EcoRII 722 CCAGG ^CC[AT]GG
EcoT14I 289 CCTTGG C^C[AT][AT]GG
HgaI 342 GCGTC GACGC(5/10)
HincII 304 GTTAAC GT[CT]^[GA]AC
HindII 304 GTTAAC GT[CT]^[GA]AC
HpaI 304 GTTAAC GTT^AAC
HphI 481 GGTGA GGTGA(8/7)
MaeI 655 CTAG C^TAG
MamI 405 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 404 AGATCC [GA]^GATC[CT]
MluNI 15 TGGCCA TGG^CCA
MscI 15 TGGCCA TGG^CCA
MslI 295 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 722 CCAGG CC^[AT]GG
NdeI 436 CATATG CA^TATG
PflMI 735 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 100 GACTC GAGTC(4/5)
ScaI 116 AGTACT AGT^ACT
ScrFI 722 CCAGG CC^[ACGT]GG
SfaNI 542 GATGC GCATC(5/9)
StyI 289 CCTTGG C^C[AT][AT]GG
Van91I 735 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 404 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 492 CCGC CCGC(-3/-1)
667 GCGG
BsaI 512 GAGACC GGTCTC(1/5)
567 GAGACC
BspMI 253 ACCTGC ACCTGC(4/8)
442 ACCTGC
Cfr13I 350 GGGCC G^G[ACGT]CC
659 GGGCC
DdeI 164 CTCAG C^T[ACGT]AG
529 CTCAG
DpnII 221 GATC ^GATC
405 GATC
EaeI 15 TGGCCA [CT]^GGCC[GA]
132 TGGCCG
MboI 221 GATC ^GATC
405 GATC
MboII 64 GAAGA GAAGA(8/7)
97 GAAGA
NdeII 221 GATC ^GATC
405 GATC
Sau3AI 221 GATC ^GATC
405 GATC
Sau96I 350 GGGCC G^G[ACGT]CC
659 GGGCC
TfiI 142 GATTC G^A[AT]TC
726 GAATC
Tsp45I 344 GTCAC ^GT[GC]AC
431 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI BsmAI BsrI BstF5I Cac8I
FokI HaeIII HinfI MaeIII MnlI
MseI MwoI NlaIII NlaIV RsaI
Tru9I Tsp509I TspRI