Restriction map for
pFN21AE6521
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# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 19 CGCG CG^CG
AflIII 256 ACATGT A^C[GA][CT]GT
AlwI 220 GATCC GGATC(4/5)
Asp700I 270 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 5 GAGCAC G[AT]GC[AT]^C
BmyI 5 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 5 GAGCAC G[AT]GC[AT]^C
BsmAI 134 GTCTC GTCTC(1/5)
BsmI 323 GCATTC GAATGC(1/-1)
Bsp1286I 5 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 256 ACATGT A^CATGT
BspMI 237 GCAGGT ACCTGC(4/8)
BsrBI 50 GAGCGG CCGCTC(-3/-3)
BssKI 395 CCTGG ^CC[ACGT]GG
BstNI 395 CCTGG CC^[AT]GG
BstUI 19 CGCG CG^CG
BstXI 388 CCAGAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 219 AGATCC [GA]^GATC[CT]
DdeI 404 CTGAG C^T[ACGT]AG
EarI 452 CTCTTC CTCTTC(1/4)
EcoRII 395 CCTGG ^CC[AT]GG
HindIII 379 AAGCTT A^AGCTT
Ksp632I 452 CTCTTC CTCTTC(1/4)
MaeIII 469 GTTAC ^GT[ACGT]AC
MflI 219 AGATCC [GA]^GATC[CT]
MvaI 395 CCTGG CC^[AT]GG
MvnI 19 CGCG CG^CG
NlaIII 257 CATG CATG^
NspI 256 ACATGT [GA]CATG^[CT]
ScrFI 395 CCTGG CC^[ACGT]GG
SpeI 9 ACTAGT A^CTAGT
TaqI 482 TCGA T^CGA
TthHB8I 482 TCGA T^CGA
XhoII 219 AGATCC [GA]^GATC[CT]
XmnI 270 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 20 GCGG CCGC(-3/-1)
52 GCGG
BbsI 499 GAAGAC GAAGAC(2/6)
228 GTCTTC
BbvI 180 GCTGC GCAGC(8/12)
247 GCTGC
BpuAI 499 GAAGAC GAAGAC(2/6)
228 GTCTTC
DpnII 220 GATC ^GATC
518 GATC
EcoRI 274 GAATTC G^AATTC
447 GAATTC
Fnu4HI 180 GCTGC GC^[ACGT]GC
247 GCTGC
HinfI 15 GATTC G^A[ACGT]TC
211 GATTC
ItaI 180 GCTGC GC^[ACGT]GC
247 GCTGC
MboI 220 GATC ^GATC
518 GATC
NdeII 220 GATC ^GATC
518 GATC
Sau3AI 220 GATC ^GATC
518 GATC
SspI 313 AATATT AAT^ATT
461 AATATT
TfiI 15 GATTC G^A[AT]TC
211 GATTC
TseI 180 GCTGC G^C[AT]GC
247 GCTGC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AcsI AluI ApoI BfaI MaeI
MboII MnlI MseI Tru9I Tsp509I