Restriction map for
pFN21AE6395
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccII AccIII AcsI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshBI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 1105 GTCTAC GT^[AC][GT]AC
AclI 1156 AACGTT AA^CGTT
AgeI 1036 ACCGGT A^CCGGT
AspHI 851 GTGCTC G[AT]GC[AT]^C
AspI 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 755 CCCGGG C^[CT]CG[GA]G
BanII 1029 GGGCTC G[GA]GC[CT]^C
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
BclI 951 TGATCA T^GATCA
BfaI 629 CTAG C^TAG
BpmI 838 CTGGAG CTGGAG(16/14)
BsaWI 1036 ACCGGT [AT]^CCGG[AT]
BsiHKAI 851 GTGCTC G[AT]GC[AT]^C
BsmFI 806 GGGAC GGGAC(10/14)
BsoBI 755 CCCGGG C^[CT]CG[GA]G
BspHI 513 TCATGA T^CATGA
BspMI 386 GCAGGT ACCTGC(4/8)
BsrDI 1098 CATTGC GCAATG(2/0)
BsrFI 1036 ACCGGT [GA]^CCGG[CT]
BsrI 1119 CCAGT ACTGG(1/-1)
BssSI 791 CACGAG CACGAG(-5/-1)
BstAPI 188 GCATCGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 637 GGTGACC G^GT[ACGT]ACC
BstF5I 1128 GGATG GGATG(2/0)
BstPI 637 GGTGACC G^GT[ACGT]ACC
Cac8I 849 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 1089 GCGC GCG^C
Cfr10I 1036 ACCGGT [GA]^CCGG[CT]
EaeI 708 TGGCCG [CT]^GGCC[GA]
EarI 833 CTCTTC CTCTTC(1/4)
EcoNI 281 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 637 GGTGACC G^GT[ACGT]ACC
EcoT14I 815 CCTTGG C^C[AT][AT]GG
FauI 142 CCCGC CCCGC(4/6)
FbaI 951 TGATCA T^GATCA
FokI 1128 GGATG GGATG(9/13)
HhaI 1089 GCGC GCG^C
HinP1I 1089 GCGC G^CGC
HindIII 445 AAGCTT A^AGCTT
Ksp632I 833 CTCTTC CTCTTC(1/4)
MaeI 629 CTAG C^TAG
MaeIII 638 GTGAC ^GT[ACGT]AC
MfeI 1224 CAATTG C^AATTG
MslI 435 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 437 CAGCTG C[AC]G^C[GT]G
MunI 1224 CAATTG C^AATTG
NspI 486 GCATGT [GA]CATG^[CT]
PflFI 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1119 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1036 ACCGGT A^CCGGT
PshAI 753 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1156 AACGTT AA^CGTT
PstI 667 CTGCAG CTGCA^G
PvuII 437 CAGCTG CAG^CTG
RcaI 513 TCATGA T^CATGA
SapI 832 GCTCTTC GCTCTTC(1/4)
SmaI 755 CCCGGG CCC^GGG
StuI 736 AGGCCT AGG^CCT
StyI 815 CCTTGG C^C[AT][AT]GG
Tsp45I 638 GTGAC ^GT[GC]AC
Tth111I 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1119 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 755 CCCGGG C^CCGGG
XmaI 755 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 99 GGTACC G^GTACC
652 GGTACC
AciI 143 CCGC CCGC(-3/-1)
1102 GCGG
AfaI 100 GTAC GT^AC
653 GTAC
Asp718I 99 GGTACC G^GTACC
652 GGTACC
AvaII 44 GGACC G^G[AT]CC
752 GGACC
BbvI 12 GCTGC GCAGC(8/12)
499 GCTGC
BseRI 612 GAGGAG GAGGAG(10/8)
794 GAGGAG
BsmAI 253 GAGAC GTCTC(1/5)
363 GAGAC
BsmI 193 GAATGC GAATGC(1/-1)
467 GCATTC
BstYI 58 GGATCT [GA]^GATC[CT]
1065 AGATCC
Cfr13I 44 GGACC G^G[ACGT]CC
752 GGACC
DsaI 452 CCACGG C^C[GA][CT]GG
1134 CCACGG
Fnu4HI 12 GCTGC GC^[ACGT]GC
499 GCTGC
HaeIII 709 GGCC GG^CC
737 GGCC
HgaI 808 GACGC GACGC(5/10)
1056 GCGTC
ItaI 12 GCTGC GC^[ACGT]GC
499 GCTGC
KpnI 99 GGTACC GGTAC^C
652 GGTACC
MaeII 597 ACGT A^CGT
1157 ACGT
MflI 58 GGATCT [GA]^GATC[CT]
1065 AGATCC
MseI 283 TTAA T^TAA
415 TTAA
PleI 321 GACTC GAGTC(4/5)
1024 GAGTC
RsaI 100 GTAC GT^AC
653 GTAC
Sau96I 44 GGACC G^G[ACGT]CC
752 GGACC
SfcI 459 CTGTAG C^T[GA][CT]AG
667 CTGCAG
SmlI 90 CTTGAG C^T[CT][GA]AG
313 CTTGAG
TaqI 191 TCGA T^CGA
600 TCGA
Tru9I 283 TTAA T^TAA
415 TTAA
TseI 12 GCTGC G^C[AT]GC
499 GCTGC
TthHB8I 191 TCGA T^CGA
600 TCGA
XhoII 58 GGATCT [GA]^GATC[CT]
1065 AGATCC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BanI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI DdeI DpnII EcoRII HapII
HinfI HpaII HphI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI TfiI Tsp509I TspRI